OBJECTIVE： To establish a method for the contents determination of 5 heavy metals in Ershiwuwei shanhu pills，and to investigate the contents of heavy metals in Ershiwuwei shanhu pills produced by 5 manufacturers from different districts of Tibet. METHODS： Ershiwuwei shanhu pills were digested by HNO3-HClO4 （4 ∶ 1，V/V）. The contents of 3 heavy metals as Cu， Pb， Cd in samples were measured by flame atomic absorption spectrometry （FAAS）. The contents of 2 heavy metals as As，Hg in samples were measured by hydride generation atomic absorption spectrometry （HG-AAS）. RESULTS： 5 kinds of heavy metals have good linear relationship in the corresponding mass concentration range （all r≥0.999 1）. The limits of detection of Cu， Pb， Cd were 0.001 6， 0.041 2， 0.036 3 mg/L， and the limits of quantitation were 0.005 3， 0.137 3， 0.121 0 mg/L； the limits of detection of As， Hg were 0.325 7， 0.692 3 μg/L， and the limits of quantitation were 1.085 7， 2.307 7 μg/L. RSDs of precision tests were ≤5.54％（n＝6）； RSDs of stability tests were all≤3.79％； RSDs of reproducibility tests were ≤3.72％. The average recovery rates were 91.34％-110.11％ （RSDs were 0.66％-6.80％，n＝6）. Results of contents determination showed that the contents of Cu in samples from 5 manufacturers were not out of limits， but the contents of Cd and Hg were all out of limits; the contents of Pb in samples from 4 manufacturers were out of limits， and the contents of As in samples from 2 manufacturers were out of limits. CONCLUSIONS： The established method has good accuracy， sensitivity， stability and reproducibility， and it is suitable for contents determination of 5 heavy metals in Ershiwuwei shanhu pills. To some extent，there is a problem of excessive heavy metals in samples from 5 manufacturers.