OBJECTIVE： To investigate the mechanism of Atractylodes macrocephala-Citrus aurantium couplet medicine on slow transit constipation （STC）. METHODS： With the aid of traditional Chinese medicine system pharmacology platform （TCMSP）， chemical components and targets of A. macrocephala and C. aurantium were retrieved. The target genes were queried through UniProt， HCBI and PubMed databases. Cytoscape 3.2.1 software was used to establish compound-targets （genes） networks and protein-protein interaction core network （PPICN） so as to screen key target. The mechanism of its action was studied through gene ontology （GO） function enrichment analysis by DAVID database and based on the Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment analysis. RESULTS： A total of 26 active compounds were screened. The compound-targets （genes） network contained 21 active compounds and 142 corresponding targets； key targets involved PTGS1， PTGS2， etc. PPICN contained 302 proteins， and key proteins involved fusion gene NTRK1， parkinson gene PARK2， antioncogene TP53， etc. The analysis of GO function enrichment obtained 741 Go item （P＜0.05）， including 457 biological process items， 131 molecular function items and 153 cell composition related items. There were 106 signal pathways （P＜0.05） in the KEGG pathway enrichment screening， involving EB virus infection pathway， viral carcinogenesis pathway， MAPK pathway and so on. CONCLUSIONS： A. macrocephala-C. aurantium couplet medicine treat STC through multiple target and multiple pathway. It provides reference for further study of their material basis and mechanism of action.