Objective To analyze the correlation between sperm DNA fragmentation index

semen routine and the artificial insemination-assisted pregnancy outcomes. Methods A total of 232 male infertility patients treated in a hospital in Hebi City from May 2019 to April 2021 were selected as the subjects. The sperm DNA fragmentation index and semen routine indicators（semen volume

sperm concentration

total sperm count

number of forward-moving sperm

number of motile sperm

and normal morphology rate） of the subjects were examined

and the artificial insemination-assisted conception outcome of the subjects was counted. The correlation between the sperm DNA fragmentation index and the conventional parameters of semen was analyzed using univariate and multifactorial analysis.Results Among 232 male infertility cases

the success rate of artificial insemination with their partners was 48.71%（113/232） and the failure rate was 51.29%（119/232）.The differences were statistically significant（P<0.05） in forward motile sperm count

motile sperm count

normal morphology rate

and sperm DNA fragmentation index among male infertility respondents with different artificial insemination-assisted conception outcomes.Pearson correlation analysis showed that sperm DNA fragmentation index was negatively correlated with forward motile sperm count

motile sperm count

and normal morphology rate（P<0.05）. The results of multivariate logistic regression analysis showed that forward motile sperm count

motile sperm count

and normal morphology rate were protective factors for artificial insemination-assisted conception success in the spouses of male infertility respondents

and sperm DNA fragmentation index was an independent risk factor for artificial insemination-assisted conception failure in the spouses of male infertility respondents（P<0.05）. Conclusion The sperm DNA fragmentation index and semen routine indicator were closely related to the outcome of artificial insemination-assisted conception

and the success rate was higher in male infertility patients who have low sperm DNA fragmentation index

high motile sperm count and motile sperm count

and high normal sperm morphology rate. This result serves as a guide for the selection of clinical artificial insemination protocol.