Study on the different extraction fractions of Agrimonia pilosa against hepatic fibrosis

LI Lin; RAO Chunyan; LEI Qingsong; HUANG Yi; LIU Huabao; ZHANG Changjiang

您当前的位置：

首页 >

文章列表页 >

Study on the different extraction fractions of Agrimonia pilosa against hepatic fibrosis

更新时间：2022-06-21

- Study on the different extraction fractions of Agrimonia pilosa against hepatic fibrosis
- China Pharmacy Vol. 33, Issue 3, (2022)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2022，
扫描看全文
LI Lin, RAO Chunyan, LEI Qingsong, et al. Study on the different extraction fractions of Agrimonia pilosa against hepatic fibrosis. [J]. China Pharmacy 33(3).(2022)
DOI：

LI Lin, RAO Chunyan, LEI Qingsong, et al. Study on the different extraction fractions of Agrimonia pilosa against hepatic fibrosis. [J]. China Pharmacy 33(3).(2022) DOI：

摘要

目的研究仙鹤草不同提取部位的抗肝纤维化作用。方法以大鼠肝星状细胞HSC-T6为研究对象，检测不同质量浓度（0.5、5、50、500、5000μg/mL，均以生药量计）的仙鹤草不同提取部位（总提取物、乙酸乙酯部位、石油醚部位和正丁醇部位）对该细胞增殖的影响（作用24、48、72h后），并计算半数抑制浓度（IC50）。采用血小板衍生生长因子（PDGF-BB）刺激HSC-T6细胞以复制肝纤维化细胞模型，采用流式细胞仪检测仙鹤草不同提取部位对HSC-T6细胞凋亡的影响，采用酶联免疫吸附试验检测细胞培养上清液中Ⅰ型胶原蛋白（Col-Ⅰ）表达水平，采用Westernblot法检测细胞中α-平滑肌肌动蛋白（α-SMA）、Col-Ⅰ、B细胞淋巴瘤2（Bcl-2）、Bcl-2相关X蛋白（Bax）、胱天蛋白酶3（caspase-3）的表达水平。结果仙鹤草总提取物、乙酸乙酯部位、石油醚部位和正丁醇部位均可显著升高HSC-T6细胞凋亡率（P＜0.01）；作用24h时，上述各部位的IC50分别为50.17、20.75、5.82、4.09μg/mL。经PDGF-BB干预后，HSC-T6细胞培养上清液中Col-Ⅰ和细胞中Col-Ⅰ、α-SMA、Bcl-2、Bax、caspase-3蛋白表达水平均显著升高（P＜0.01）；经仙鹤草不同提取部位干预后，HSC-T6细胞培养上清液或细胞中上述蛋白表达水平大部分均较PDGF-BB组显著逆转（P＜0.05或P＜0.01），其中仙鹤草正丁醇部位干预作用最为显著。结论仙鹤草不同提取部位均可抑制HSC-T6细胞增殖，并诱导其凋亡；正丁醇部位可能是仙鹤草发挥抗肝纤维化作用的有效部位。

Abstract

OBJECTIVE To study different extraction fractions of Agrimonia pilosa against h epatic fibrosis. METHODS Using hepatic stellate cells HSC-T 6 of rats as objects ，the effects of different extraction fractions （total extract ，ethyl acetate fraction ， petroleum ether fraction and n-butanol fraction ）with different concentrations （0.5，5，50，500，5 000 μg/mL，calculated by raw drug）of A. pilosa on the proliferation of HSC-T 6 cells were detected （after treated for 24，48，72 h）；median inhibition concentration（IC50）was also caculated. Platelet-derived growth factor （PDGF-BB）was used to induce the activation of HSC-T 6 cells to establish hepatic fibrosis cell model. Flow cytometry was used to detect the effects of different extraction fractions of A. pilosa on apoptosis of HSC-T 6 cells. The expression of collagen Ⅰ（Col-Ⅰ）in the supernatant was detected by enzyme linked immunosorbent assay. The expressions of α-smooth muscle actin （α-SMA），Col-Ⅰ，B-cell lymphoma- 2（Bcl-2），Bcl-2-associated X protein （Bax）and caspase- 3 were detected by Western blot assay. RESULTS Total extract ，ethyl acetate fraction ，petroleum ether fraction and n-butanol fraction of A. pilosa could significantly increase the apoptotic rate of HSC-T 6 cells（P＜0.01）. After treated for 24 h，IC50 of above fractions were 50.17，20.75，5.82，4.09 μg/mL，respectively. After intervened with PDGF-BB ，the expression of Col- Ⅰ in supernatant of HSC-T 6 cells as well as protein expression of Col- Ⅰ，α-SMA，Bcl-2，Bax and caspase- 3 in HSC-T6 cells were increased significantly （P＜0.01）. After intervened with different extraction fractions of A. pilosa ，most of the expressions of above proteins in HSC-T 6 cell culture supernatant or cells were significantly reversed compared with PDGF-BB group （P＜0.05 or P＜0.01）， and the intervention effect of n-butanol fraction of A. pilosa was the most significant. CONCLUSIONS Different extraction fractions of A. pilosa can inhibite the proliferation of HSC-T 6 cells and induce their apoptosis；n-butanol fraction from A. pilosa may be an effective fraction to exert the effect of anti-hepatic fibrosis.

关键词

仙鹤草肝纤维化细胞凋亡有效部位

Keywords

references

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on Improvement Effects of Mongolian Medicine Eligen- 7 on Hepatic Fibrosis and Its Mechanism

Inhibitory effect and mechanism of total flavonoids from Melicope pteleifolia on transplanted tumor of colorectal cancer in nude mice

Study on inhibitory effects and mechanism of silymarin on glioma in vivo and in vitro

Comparison of anti-ischemic stroke effect of different extraction parts from Gastrodia elata

Study on the protective mechanism of Bawei chenxiang powder containing serum on H9c2 cells injured by oxygen-glucose deprivation

Related Author

YAN Yuxin

ZHANG Chunyan

GAO Xiaoyang

MA Yuehong

LIU Yi

AN Zhenxiang

HE Yuanli

GAO Ying

Related Institution

First Clinical Medical College， Guizhou University of Traditional Chinese Medicine

Bijie Medical College

Dept. of Gastroenterology， the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine

Dept. of Neurosurgery， the First Affiliated Hospital of Hebei North University

College of Traditional Chinese Medicine， Yunnan University of Traditional Chinese Medicine

⁰