Activation levels of NLRP3 inflammasomes in the ventrolateral periaqueductal gray of rats with neuropathic pain

FU Dan-hong; PENG Mei-jing; HUANG Hai-ying; GAO Xian-wei

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Activation levels of NLRP3 inflammasomes in the ventrolateral periaqueductal gray of rats with neuropathic pain

更新时间：2022-06-24

- Activation levels of NLRP3 inflammasomes in the ventrolateral periaqueductal gray of rats with neuropathic pain
- null Issue 2, Pages: 108(2022)
- 作者机构：
  
  1. 福建中医药大学附属康复医院麻醉科,福建,福州,350003
  2. 福建省康复技术重点实验室,福建,福州,350003
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FU Dan-hong, PENG Mei-jing, HUANG Hai-ying, et al. Activation levels of NLRP3 inflammasomes in the ventrolateral periaqueductal gray of rats with neuropathic pain. [J]. null (2):108(2022)
DOI：

FU Dan-hong, PENG Mei-jing, HUANG Hai-ying, et al. Activation levels of NLRP3 inflammasomes in the ventrolateral periaqueductal gray of rats with neuropathic pain. [J]. null (2):108(2022) DOI：

摘要

目的探讨核苷酸结合寡聚化结构域样受体蛋白3（NLRP3）炎症小体在神经病理性疼痛大鼠中脑导水管周围灰质腹外侧区（vlPAG）的表达水平及其作用。方法选取SPF级SD大鼠20只，采用随机数字表法分为假手术组(Sham组)与慢性坐骨神经压迫性损伤组(CCI组)，每组10只。于手术前后测定2组机械痛阈与热痛阈；Western blot检测2组NLRP3炎症小体各组分蛋白表达水平；RT-PCR检测2组NLRP3炎症小体各组分基因mRNA表达水平；酶联免疫吸附实验（ELISA）检测2组炎症因子白介素-lβ（IL-1β）及白介素-18（IL-18）表达水平；免疫荧光检测2组中NLRP3炎症小体在vlPAG的分布情况，并与小胶质细胞标志物IBA1进行共定位分析。结果与Sham组相比，CCI组术后3 d、7 d、14 d、21 d机械痛阈与热痛阈均明显降低（P＜0.001）；与Sham组相比，CCI组核心蛋白NLRP3、效应蛋白Caspase-1 p10及前体蛋白pro-Caspase-1表达水平均明显升高（P＜0.001）；与Sham组相比，CCI组NLRP3（P＜0.001）、Caspase-1（P＜0.001）及IL-1β（P＜0.01）mRNA表达水平均明显升高，炎症因子IL-1β（P＜0.001）与IL-18（P＜0.01）表达水平也明显升高；与Sham组相比，CCI组在vlPAG的NLRP3阳性细胞数增加，且与IBA1存在共表达。结论神经病理性疼痛大鼠vlPAG中NLRP3炎症小体被激活且表达水平增高，提示NLRP3炎症小体激活水平与神经病理性疼痛大鼠痛阈下降相关。

Abstract

关键词

神经病理性疼痛中脑导水管周围灰质腹外侧区NLRP3炎症小体炎症因子慢性坐骨神经压

Keywords

neuropathic painventrolateral periaqueductal grayNLRP3 inflammasomesinflammatory factorschronic constriction

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