miR-129 inhibits proliferation,migration and invasion of breast cancer cells by targeting Twist1

HUANG Fang-jie; SU Shi-xin; CHENG Yu-chang

您当前的位置：

首页 >

文章列表页 >

miR-129 inhibits proliferation,migration and invasion of breast cancer cells by targeting Twist1

更新时间：2022-06-24

- miR-129 inhibits proliferation,migration and invasion of breast cancer cells by targeting Twist1
- null Issue 2, Pages: 119(2022)
- 作者机构：
  
  1. 中国科学院大学深圳医院(光明)甲乳外科,广东,深圳,518000
  2. 中国科学院大学深圳医院(光明)肾内科,广东,深圳,518000
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
HUANG Fang-jie, SU Shi-xin, CHENG Yu-chang. miR-129 inhibits proliferation,migration and invasion of breast cancer cells by targeting Twist1. [J]. null (2):119(2022)
DOI：

HUANG Fang-jie, SU Shi-xin, CHENG Yu-chang. miR-129 inhibits proliferation,migration and invasion of breast cancer cells by targeting Twist1. [J]. null (2):119(2022) DOI：

摘要

目的研究miR-129对乳腺癌细胞增殖、迁移及侵袭的影响及其作用机制。方法选取乳腺癌SKBR3、AU565细胞系，建立过表达与敲低miR-129细胞系，将细胞分为Control-mimic组、miR-129-mimic组和miR-129-mimic+miR-mimic-shRNA组。采用RT-PCR检测SKBR3、AU565细胞系中miR-129的表达水平，CCK-8法检测SKBR3、AU565细胞系的增殖能力，通过划痕实验及Transwell实验测定SKBR3、AU565细胞系的迁移及侵袭能力。采用生物信息学和双荧光素酶报告系统检测miR-129与Twist1的互补结合位点，采用Western blot对miR-129是否是Twist1的转录靶点进行分析验证。结果 miR-129-mimic组SKBR3和AU565细胞中miR-129的表达水平显著高于Control-mimic组（P＜0.05）。miR-129-mimic组SKBR3和AU565细胞的增殖能力明显低于Control-mimic组（P＜0.01）

而miR-129-mimic+miR-mimic-shRNA组逆转了miR-129过表达导致的细胞增殖能力降低。与Control-mimic组比较，miR-129-mimic组中SKBR3和AU565细胞的迁移及侵袭能力明显下降（P＜0.01）。生物信息学软件分析显示，miR-129与Twist1的3’-UTR端有互补结合位点，且双荧光素酶试验结果显示，miR-129-mimic组中WT-3’Twist-UTR的荧光素酶活性明显低于Control-mimic组（P＜0.01）；而miR-129-mimic组中Mut-3’Twist-UTR的荧光素酶活性与Control-mimic组比较，差异无统计学意义（P＞0.05）；Western blot结果显示，与Control-mimic组比较，miR-129-mimic组SKBR3和AU565细胞中Twist1蛋白水平明显下降（P＜0.01）。结论 miR-129可通过靶向调节Twist1抑制乳腺癌细胞增殖、迁移和侵袭，是乳腺癌诊断及治疗的潜在靶点。

Abstract

关键词

miR-129乳腺癌增殖迁移侵袭

Keywords

miR-129breast cancerproliferationmigrationinvasion

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

hsa_circ_0011946 regulates the proliferation, migration and invasion of cervical cancer cells via targeting miR-767-3p

Expression and significance of CDCA3 in epithelial ovarian cancer

Effect of silencing PROX1 gene on TPC-1 cells of papillary thyroid carcinoma

Mechanism of lncRNA MIR4435-2HG targeting miR-376a-3p to regulate the biological behavior of cholangiocarcinoma cells

Ferulic acid inhibits the progression of T-cell acute lymphoblastic leukemia by regulating PTEN/PI3K/AKT signaling pathway

Related Author

LI Wen-ya

XIE Cheng

PENG Li

LI Miao

JIN Hai-hong

FU Jing-jing

HAN Kun

GONG Shan

Related Institution

Department of Nephrology， Xiantao First People's Hospital Affiliated to Yangtze University

Department of Obstetrics and Gynecology， Xiantao First People's Hospital Affiliated to Yangtze University

Department of Gynecology， Qinhuangdao First Hospital

Department of Pathology,Baoji Maternal and Child Health Care Hospital

Department of Comprehensive Surgery,Baoji Maternal and Child Health Care Hospital

⁰