Effects of miR-483 targeting DLC1 on the proliferation,migration and invasion of triple negative breast cancer cells

LI Pi-song; CHEN Hong-shen; CHENG Xiao-yu; ZHU Xiao-yu

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Effects of miR-483 targeting DLC1 on the proliferation,migration and invasion of triple negative breast cancer cells

更新时间：2022-06-24

- Effects of miR-483 targeting DLC1 on the proliferation,migration and invasion of triple negative breast cancer cells
- null Issue 4, Pages: 305(2022)
- 作者机构：
  
  大连大学附属中山医院乳甲外科,辽宁,大连,116000
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LI Pi-song, CHEN Hong-shen, CHENG Xiao-yu, et al. Effects of miR-483 targeting DLC1 on the proliferation,migration and invasion of triple negative breast cancer cells. [J]. null (4):305(2022)
DOI：

LI Pi-song, CHEN Hong-shen, CHENG Xiao-yu, et al. Effects of miR-483 targeting DLC1 on the proliferation,migration and invasion of triple negative breast cancer cells. [J]. null (4):305(2022) DOI：

摘要

目的探讨miR-483靶向调控肝癌缺失基因1（DLC1）对三阴性乳腺癌细胞增殖、迁移和侵袭的影响。方法将MDA-MB-231乳腺癌细胞分为对照组、miR-NC组、miR-483 inhibitor组、miR-483 mimics组，各组细胞设6个平行样，培养72 h。采用MTT法检测各组细胞活力，结晶紫染色检测细胞单克隆形成数目，流式细胞仪测定细胞凋亡水平，Transwell小室测定细胞迁移与侵袭水平，RT-qPCR法及蛋白印迹法测定细胞miR-483、DLC1 mRNA和蛋白表达水平。结果与对照组、miR-NC组比较，miR-483 inhibitor组细胞OD值、存活率、单克隆形成数目、穿膜数、miR-483 mRNA表达水平降低/减少（P<0.05），miR-483 mimics组细胞OD值、存活率、单克隆形成数目、穿膜数、miR-483 mRNA表达水平升高/增加（P<0.05）；与miR-483 inhibitor组比较，miR-483 mimics组OD值、存活率、单克隆形成数目、穿膜数、miR-483 mRNA表达水平升高/增加（P<0.05）。与对照组、miR-NC组比较，miR-483 inhibitor组凋亡率、DLC1 mRNA和蛋白表达水平升高（P<0.05），miR-483 mimics组凋亡率、DLC1 mRNA和蛋白表达水平降低（P<0.05）；与miR-483 inhibitor组比较，miR-483 mimics组凋亡率、DLC1 mRNA和蛋白表达水平降低（P<0.05）。结论 miR-483过表达能促进三阴性乳腺癌细胞增殖、侵袭，抑制其凋亡，其机制可能与miR-483抑制DLC1表达有关。

Abstract

关键词

miR-483肝癌缺失基因1三阴性乳腺癌细胞增殖细胞迁移细胞侵袭

Keywords

miR-483deleted in liver cancer 1triple negative breast cancercell proliferationcell migrationcell invasion

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