Objective: To study the pharmacological effects of chloramphenicol prednisone liniment on delayed hypersensitivity in mice. Methods: Experimental animal model of delayed hypersensitivity in mice was induced by dinitrochlorobenzene (DNCB) acetone solution

the chloramphenicol prednisone liniment high dose group

medium dose group

low dose group

momethasone cream group

model control group

matrix control group and blank control group for experimental animals were established

and the effects on delayed hypersensitivity were observed. Antipruritic threshold was detected by histamine phosphate. Results: Compared with the model group

there were significant differences among the chloramphenicol prednisone liniment high dose group

medium dose group and low dose group in the index of ear swelling (P<0.05); there was no statistically significant difference between the low dose group and the model group (P>0.05). Compared with the model group

the difference of antipruritic threshold of phosphate histamine in the chloramphenicol prednisone liniment high

medium and low dose group was statistically significant (P<0.05). Conclusion: The topical application of chloramphenicol prednisone liniment has good anti-hypersensitivity and antipruritic effects in mice

which is suitable for clinical treatment of skin lesions with delayed hypersensitivity.