Objective：To summarize the main point and experience of Da Vinci robot laparoendoscopic single-site（R-LESS） on nurs－ing coordination in the comprehensive operative stage of endometrial cancer. Methods：Clinical data and nursing cooperation during operation of nine cases of Da Vinci R-LESS endometrial cancer surgery were retrospectively analyzed. Results：Operations of nine patients were successfully completed and the postoperative recovery was good. There were no medical and nursing complications dur－ing and after the operation. Conclusion：Professional robotic surgery team，thorough surgical plan，sufficient nursing preparation and skilled and smooth intraoperative nursing coordination ensure the successful implementation of R-LESS surgery.