Objective：To retrospectively analyze the difference of 2019 novel coronavirus（2019-nCoV） nucleic acid test results among individuals of different age and gender in Hubei area，and provide prevention and treatment strategies of coronavirus disease 2019（COVID-19）. Methods：A total of 84 611 examinees were collected and divided into 9 groups by age：0-，10-，20-，30-，40-，50-，60-，70-，and 80-. Each age group was divided into male group and female group. The results of 2019-nCoV nucleic acid test in each group were statistically analyzed. Results：Positive rate of 2019-nCoV nucleic acid test was 5.33% in all examinees，among which only N gene positive results accounted for 66.42%. Positive rates of 2019-nCoV nucleic acid test were increased gradually with age，and reached the peak in 60- years old group. Positive rate of 2019-nCoV nucleic acid test in entire female group was 6.21% and signifi－cantly higher than that in entire male group（4.64%），and the differences were caused mainly by 20-60 years old female groups which had obviously higher positive rates of 2019-nCoV nucleic acid test than same age male groups（P＜0.05）. Conclusion：Positive rates of 2019-nCoV nucleic acid test are related to age and gender. The female aged 20-60 are more susceptible to infection than men. The positive rates of 2019-nCoV nucleic acid test were gradually increased with age before 70 years old.