Objective：To measure the morphology of normal patella with the three-dimensional software，so as to provide anatomical parameters for surgical treatment of patella fracture and improving guide devices. Methods：A total of 458 cases of knee DICOM for－mat data from August 2016 to December 2018 were collected. After excluding substandard samples，94 cases of normal patella were selected，including 49 cases of male and 45 cases of female. All the DICOM data were put into Mimics software for reconstruction and measurement，and patellar pole height，patellar height，patellar width，patellar thickness，inner 1/3 thickness and outer 1/3 thickness of different gender were measured in groups. Results：Patellar pole height，patellar height，patellar width，patellar thickness，inner 1/3 thickness and outer 1/3 thickness in the male group were （46.87±2.32），（31.42±1.83），（44.83±2.42），（21.86±1.87），（19.62±1.76） and （15.38±1.89） mm，respectively；in the female group were （42.38±1.98），（28.06±2.01），（39.12±2.03），（19.47±1.93），（17.31±1.93），（14.01±1.72） mm，respectively，with statistically significant differences among those parameters. Male patella was larger than female patella，and inner 1/3 thickness was less than outer 1/3 thickness. A guide device suitable to patella tension band was successfully designed. Conclusion：The measurement of patella anatomical parameters provides anatomical basis for treating patella fracture and guide device of patella tension band makes fixation of patella fracture more accurate and convenient.