Isolation and identification of liver sinusoidal endothelial cells（LSECs） are the bases to study their physiological functions，and immunophenotype is the key to this work. However，the lack of specific immunophenotype on LSECs is the major challenge in their researches. The immunophenotype of LSECs is transformed with the functional changes of the liver at different stages，influenced by the metabolic microenvironment in hepatic lobules and regulated by cytokines，with the property of heterogeneity. This article reviewed the expression，physiological functions，heterogeneity and causes of LSECs’ immunophenotype and briefly analyzed the research advances of the LSECs’ immunophenotype.