The influence of Zhilong Huoxue Tongyu Capsule on blood lipid level and MMP-2 and MMP-9 in rabbits with carotid atherosclerosis

HAN Mei; YANG Si-jin; JIANG Yan; LIU Meng-nan

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The influence of Zhilong Huoxue Tongyu Capsule on blood lipid level and MMP-2 and MMP-9 in rabbits with carotid atherosclerosis

更新时间：2023-04-14

- The influence of Zhilong Huoxue Tongyu Capsule on blood lipid level and MMP-2 and MMP-9 in rabbits with carotid atherosclerosis
- Journal of Southwest Medical University Vol. 44, Issue 3, Pages: 197-203(2021)
- 作者机构：
  
  西南医科大学附属中医医院心脑病科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5
- Published：2021，
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[1]韩梅,杨思进,江燕,刘孟楠.蛭龙活血通瘀胶囊对颈动脉粥样硬化模型兔血脂代谢及MMP-2、MMP-9的影响[J].西南医科大学学报,2021,44(03):197-203.

HAN Mei, YANG Si-jin, JIANG Yan, et al. The influence of Zhilong Huoxue Tongyu Capsule on blood lipid level and MMP-2 and MMP-9 in rabbits with carotid atherosclerosis. [J]. Journal of Southwest Medical University 44(3):197-203(2021)
[1]韩梅,杨思进,江燕,刘孟楠.蛭龙活血通瘀胶囊对颈动脉粥样硬化模型兔血脂代谢及MMP-2、MMP-9的影响[J].西南医科大学学报,2021,44(03):197-203. DOI：

HAN Mei, YANG Si-jin, JIANG Yan, et al. The influence of Zhilong Huoxue Tongyu Capsule on blood lipid level and MMP-2 and MMP-9 in rabbits with carotid atherosclerosis. [J]. Journal of Southwest Medical University 44(3):197-203(2021) DOI：

摘要

目的通过观察蛭龙活血通瘀胶囊对颈动脉粥样硬化模型兔血脂、基质金属蛋白酶-2（matrix metalloproteinase-2

MMP-2）及基质金属蛋白酶-9（matrix metalloproteinase-9

MMP-9）表达的影响

进而评估该药物对颈动脉粥样硬化的改善作用。方法将50只新西兰大白兔适应性喂养1周后按随机数字表法平分为5组

即正常对照组、模型组、蛭龙活血通瘀胶囊组、阿托伐他汀钙片组、蛭龙活血通瘀胶囊+阿托伐他汀钙片混合用药组。采用高脂饲料饮食+颈动脉内膜空气干燥术造模。术后1周正式给药

分别予以:蛭龙活血通瘀胶囊组3.12 g生药/kg/d;阿托伐他汀钙片组0.51 mg/kg/d;混合用药组3.12 g生药/kg/d+0.51 mg/kg/d。各组实验兔药物干预4周后采集血清标本测定其血脂水平

采集右侧颈总动脉制备病理切片并观察各标本组织病理改变

利用Western Blot法测定各颈动脉组织标本中MMP-2、MMP-9的蛋白活性。结果血脂水平:模型组较正常对照组各血脂指标均明显升高

差异具有统计学意义（P <0.05）;与模型组比较各用药组实验兔血脂水平均有不同程度降低

差异具有统计学意义。MMP-2、MMP-9蛋白活性:与正常组相比

模型组MMP-2、MMP-9蛋白表达明显增加

差异具有统计学意义（P <0.05）;经蛭龙活血通瘀胶囊或阿托伐他汀钙片单独干预后

MMP-2蛋白水平有所下降

与模型组相比

差异具有统计学意义（P <0.05）;MMP-9蛋白水平在蛭龙活血通瘀胶囊组显著降低（P <0.05）;当中西药物组联合干预后

上述两种蛋白变化趋势均较单药物组更为明显（P <0.05）。结论蛭龙活血通瘀胶囊可在一定程度上改善颈动脉粥样硬化血脂水平

抑制或减轻MMP-2、MMP-9的表达

稳定易损斑块

从而起到保护颈动脉粥样硬化的作用。

Abstract

Objective To observe the effects of Zhilong Huoxue Tongyu Capsules on the levels of blood lipids and the expression of matrix metalloproteinase-2（MMP-2）and matrix metalloproteinase-9（MMP-9）in a rabbit model of carotid atherosclerosis

and to evaluate the protective effect of the drug against carotid atherosclerosis.Methods After adaptive feeding for 1 week

50 New Zealand white rabbits were randomly divided into five groups:normal control group

model group

Zhilong Huoxue Tongyu Capsule group

atorvastatin calcium tablet group

and Zhilong Huoxue Tongyu Capsule combined with atorvastatin calcium tablet group. A rabbit model of carotid atherosclerosis was established by using a high-fat diet combined with air desiccation of the carotid intima. One week after surgery

the Zhilong Huoxue Tongyu Capsule group was given the crude drug 3.12 g/kg/d

the atorvastatin calcium tablet group was given atorvastatin calcium 0.51 mg/kg/d

and the combination group was treated with the crude drug 3.12 g/kg/d plus atorvastatin calcium 0.51 mg/kg/d. After 4 weeks of drug intervention

blood samples were collected to measure the levels of blood lipids; the right carotid arteries were collected to prepare pathological sections and observe the pathological changes of each specimen

and Western blot was used to determine the activity of MMP-2 and MMP-9 in each carotid artery specimen. Results Compared with the normal control group

the model group showed significantly increased levels of all blood lipids and significantly increased expression of MMP-2 and MMP-9（all P < 0.05）. Compared with the model group

all drug treatment groups showed significantly decreased levels of blood lipids（P < 0.05）. Both single-drug groups had significantly reduced expression of MMP-2（P <0.05）; the Zhilong Huoxue Tongyu Capsule group exhibited significantly reduced expression of MMP-9（P < 0.05）

but there was no significant change in the atorvastatin calcium tablet group. The combination group showed significantly greater reductions in MMP-2 and MMP-9 levels than the single-drug groups（P < 0.05）. Conclusion Zhilong Huoxue Tongyu Capsules can reduce blood lipid levels

inhibit the expression of MMP-2 and MMP-9 in carotid atherosclerosis

and stabilize vulnerable plaques

showing a protective effect against carotid atherosclerosis.

关键词

蛭龙活血通瘀胶囊颈动脉粥样硬化兔血脂MMP-2MMP-9

Keywords

Zhilong Huoxue Tongyu capsuleCarotid atherosclerosisRabbitBlood lipidsMatrix metalloproteinase-2Matrix metalloproteinase-9

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