Effect of transient receptor potential melastatin 7 channel on the proliferation and migration of human buccal mucosal fibroblasts

LI Aidi; NIE Minhai; ZUO Dongchuan; RAO Yong; ZENG Jin

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Effect of transient receptor potential melastatin 7 channel on the proliferation and migration of human buccal mucosal fibroblasts

更新时间：2023-04-14

- Effect of transient receptor potential melastatin 7 channel on the proliferation and migration of human buccal mucosal fibroblasts
- Journal of Southwest Medical University Vol. 43, Issue 2, Pages: 135-139(2020)
- 作者机构：
  
  1. 西南医科大学附属口腔医院牙周黍膜科
  2. 西南医科大学心血管医学研究所
  3. 附属口腔医院正畸科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.2
- Published：2020，
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[1]李爱迪,聂敏海,左东川,饶勇,曾锦.TRPM7通道对人口腔颊黏膜成纤维细胞增殖和迁移的影响[J].西南医科大学学报,2020,43(02):135-139.

LI Aidi, NIE Minhai, ZUO Dongchuan, et al. Effect of transient receptor potential melastatin 7 channel on the proliferation and migration of human buccal mucosal fibroblasts. [J]. Journal of Southwest Medical University 43(2):135-139(2020)
[1]李爱迪,聂敏海,左东川,饶勇,曾锦.TRPM7通道对人口腔颊黏膜成纤维细胞增殖和迁移的影响[J].西南医科大学学报,2020,43(02):135-139. DOI：

LI Aidi, NIE Minhai, ZUO Dongchuan, et al. Effect of transient receptor potential melastatin 7 channel on the proliferation and migration of human buccal mucosal fibroblasts. [J]. Journal of Southwest Medical University 43(2):135-139(2020) DOI：

摘要

目的:探讨瞬时受体电位离子通道TRPM7 （transient receptor potential melastatin-7）在人口腔颊黏膜成纤维细胞增殖和迁移中的作用。方法:逆转录聚合酶链式反应、免疫荧光法及全细胞膜片钳检测成纤维细胞中TRPM7的表达。MTT和细胞划痕实验分别检测应用2-氨基乙基联苯基硼酸酯（2-APB）或shRNA抑制TRPM7通道对成纤维细胞增殖和迁移的影响。结果:人口腔颊黏膜成纤维细胞功能性表达TRPM7通道。TRPM7 shRNA作用48 h后成功阻断TRPM7通道

500μmol/L2-APB可抑制其表达

两组与对照组相比

膜片钳技术记录到TRPM7样电流明显减弱

在24 h后均明显抑制成纤维细胞的增殖和迁移能力

差异有统计学意义（P <0.05）。结论:TRPM7通道参与人口腔颊黏膜成纤维细胞增殖以及迁移能力的调控。

Abstract

Objective: To investigate the role of transient receptor potential melastatin 7（TRPM7） in the prolifer-ation and migration of human buccal mucosal fibroblasts. Methods: Reverse transcription-polymerase chain reac-tion

immunofluorescence method

and whole-cell patch clamp were used to determine the expression of TRPM7 infibroblasts. MTT and wound-healing assays were used to examine the effects of inhibiting TRPM7 channels using2-aminoethyl diphenylborinate（2-APB） or shRNA on fibroblast proliferation and migration. Results: TRPM7 chan-nels were functionally expressed in human buccal mucosal fibroblasts. TRPM7 channels were successfully blockedafter 48 h of TRPM7 shRNA treatment

and their expression was inhibited by 500 μmol/L of 2-APB. Comparedwith the control group

the TRPM7-like currents were significantly decreased in the two experimental groups accord-ing to patch-clamp recording

and the proliferation and migration abilities of fibroblasts were significantly sup-pressed after 24 h of treatments（P < 0.05）. Conclusion: The TRPM7 channel is involved in the regulation of prolif-eration and migration of human buccal mucosal fibroblasts.

关键词

成纤维细胞TRPM7细胞增殖细胞迁移

Keywords

FibroblastsTransient receptor potential melastatin 7Cell proliferationCell migration

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Related Institution

Department of Health Management Center，the Affiliated Hospital of Southwest Medical University

Department of Otolaryngology Head and Neck Surgery，the Affiliated Hospital of Southwest Medical University

Department of Laboratory Medicine，the Affiliated T.C.M Hospital of Southwest Medical University

partment of Rheumatology and Immunology，the Affiliated Hospital of Southwest Medical University

The Key Laboratory of Medical Electrophysiology，Ministry of Education，Medical Electrophysiological Key Lab of Sichuan Province， Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease of Sichuan Province，Institute of Cardiovascular Research，Southwest Medical University

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