Mechanism of moist exposed burn ointment promoting wound repair in diabetic mice based on the GRP78/PERK/CHOP signaling pathway mediated by endoplasmic reticulum stress

Fan Kunyue; Li Jiehui; Lao Riling; Gan Liyan; Wang Yaya; Ni Baoling; Wang Li

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Mechanism of moist exposed burn ointment promoting wound repair in diabetic mice based on the GRP78/PERK/CHOP signaling pathway mediated by endoplasmic reticulum stress

更新时间：2026-02-27

- Mechanism of moist exposed burn ointment promoting wound repair in diabetic mice based on the GRP78/PERK/CHOP signaling pathway mediated by endoplasmic reticulum stress
- Journal of Chongqing Medical University Pages: 1-8(2026)
- 作者机构：
  
  1.广西中医药大学第一临床医学院，南宁 530021
  2.广西中医药大学第一附属医院创面修复外科，南宁 530023
  3.广西壮族自治区中医药研究院，南宁 530023
- 作者简介：
- 基金信息：
- DOI：
  CLC： R641
- Received：11 December 2025，
  
  Revised：2026-01-06，
  
  Accepted：20 January 2026，
  
  Online First：27 February 2026，
- 稿件说明：
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Fan Kunyue,Li Jiehui,Lao Riling,et al.Mechanism of moist exposed burn ointment promoting wound repair in diabetic mice based on the GRP78/PERK/CHOP signaling pathway mediated by endoplasmic reticulum stress[J].Journal of Chongqing Medical University,
DOI：

Fan Kunyue,Li Jiehui,Lao Riling,et al.Mechanism of moist exposed burn ointment promoting wound repair in diabetic mice based on the GRP78/PERK/CHOP signaling pathway mediated by endoplasmic reticulum stress[J].Journal of Chongqing Medical University, DOI：10.13406/j.cnki.cyxb..

摘要

目的

探讨湿润烧伤膏（moist exposed burn ointment，MEBO）对糖尿病创面中葡萄糖调节蛋白78（glucose-regulated protein 78，GRP78）/蛋白激酶R样内质网激酶（protein kinase R-like endoplasmic reticulum kinase，PERK）/CCAAT/增强子结合蛋白同源蛋白（CCAAT/enhancer binding protein homologous protein，CHOP）信号通路（即GRP78/PERK/CHOP信号通路）表达的影响。

方法

选取27只自发性糖尿病db/db雄性小鼠随机分为模型组、MEBO组和重组牛碱性成纤维细胞生长因子凝胶（recombinant bovine basic fibroblast growth factor，rb-bFGF）组，再选取9只同品系但不发病的杂合db/m雄性小鼠作为对照组，随后MEBO组和rb-bFGF组分别采用MEBO和rb-bFGF进行外用干预给药，对照组及模型组采取矿脂（凡士林）进行干预，每日观察并计算干预给药后3、6、12 d的创面愈合情况；应用Western blot法及免疫组化法观察3、6、12 d各组创面组织中GRP78、PERK和CHOP的蛋白表达情况。

结果

①创面干预给药的3 d，对照组创面愈合率明显高于模型组，差异有统计学意义（

0.01）；创面干预给药的6、12 d，对照组、MEBO组和rb-bFGF组创面愈合率明显高于模型组（

0.01）。②创面干预给药的3 d，各组创面组织中GRP78、PERK和CHOP蛋白表达差异无统计学意义（

0.05）；创面干预给药的6、12 d，模型组创面组织中GRP78、PERK和CHOP蛋白表达均明显高于对照组，MEBO组和rb-bFGF组创面组织中的GRP78、PERK和CHOP蛋白表达均明显低于模型组（

0.05）。

结论

MEBO可能通过抑制GRP78/PERK/CHOP信号通路的表达从而抑制过度的内质网应激（endoplasmic reticulum stress，ERS），促进糖尿病创面愈合。

Abstract

Objective

To investigate the effect of moist exposed burn ointment（MEBO） on the expression of the glucose-regulated protein 78（GRP78）/protein kinase R-like endoplasmic reticulum kinase（PERK）/CCAAT/enhancer-binding protein homologous protein（CHOP） signaling pathway in diabetic wounds.

Methods

A total of 27 spontaneous diabetic db/db male mice were randomly divided into model group，MEBO group，and recombinant bovine basic fibroblast growth factor（rb-bFGF） gel group，and then 9 heterozygous db/m male mice of the same strain without disease were selected as control group. The mice in the MEBO group and the rb-bFGF group were given intervention with the external application of MEBO and rb-bFGF，respectively，and those in the control group and the model group were given Vaseline for intervention. The mice were observed daily，and wound healing was calculated on days 3，6，and 12 of intervention. Western blot and immunohistochemistry were used to measure the protein expression levels of GRP78，PERK and CHOP in the wound tissue of each group on days 3，6，and 12.

Results

On day 3 of wound intervention，the control group had a significantly higher wound healing rate than the model group（

0.01），and on days 6 and 12 of wound intervention，the control group，the MEBO group，and the rb-bFGF group had a significantly higher wound healing rate than the model group（

0.01）. On day 3 of wound intervention，there were no significant differences in the protein expression levels of GRP78，PERK，and CHOP in wound tissue between groups（

0.05），and on days 6 and 12 of wound intervention，the model group had significantly higher protein expression levels of GRP78，PERK，and CHOP in wound tissue than the control group，and the MEBO group and the rb-bFGF group had significantly lower protein expression levels of GRP78，PERK，and CHOP than the model group（

0.05）.

Conclusion

MEBO may inhibit excessive endoplasmic reticulum stress and promote diabetic wound healing by inhibiting the expression of the GRP78/PERK/CHOP signaling pathway.

关键词

Keywords

references

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