Effect of Antisense Gene to Human Tankyrase 1 on the Morphology of SGC-7901 Gastric Cancer Cell after Transfection

YANG Meng-hua1 YANG Shi-ming2 FANG Dian-chun2 LUO Yuan-hui2

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Effect of Antisense Gene to Human Tankyrase 1 on the Morphology of SGC-7901 Gastric Cancer Cell after Transfection

更新时间：2021-08-23

- Effect of Antisense Gene to Human Tankyrase 1 on the Morphology of SGC-7901 Gastric Cancer Cell after Transfection
- Health Medicine Research and Practice Vol. 8, Issue 2, Pages: 4-6(2011)
- 作者机构：
  
  1. 西南大学医院
  2. 第三军医大学西南医院全军消化专科中心
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.2
- Published：2011，
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[1]杨孟华,杨仕明,房殿春,罗元辉.人tankyrase 1反义基因转染对SGC-7901胃癌细胞形态学的影响[J].保健医学研究与实践,2011,8(02):4-6.

YANG Meng-hua1 YANG Shi-ming2 FANG Dian-chun2 LUO Yuan-hui2. Effect of Antisense Gene to Human Tankyrase 1 on the Morphology of SGC-7901 Gastric Cancer Cell after Transfection. [J]. Health Medicine Research and Practice 8(2):4-6(2011)
[1]杨孟华,杨仕明,房殿春,罗元辉.人tankyrase 1反义基因转染对SGC-7901胃癌细胞形态学的影响[J].保健医学研究与实践,2011,8(02):4-6. DOI：

YANG Meng-hua1 YANG Shi-ming2 FANG Dian-chun2 LUO Yuan-hui2. Effect of Antisense Gene to Human Tankyrase 1 on the Morphology of SGC-7901 Gastric Cancer Cell after Transfection. [J]. Health Medicine Research and Practice 8(2):4-6(2011) DOI：

摘要

目的探讨反义tankyrase 1基因转染对SGC-7901胃癌细胞形态学的影响。方法构建人tankyrase 1基因反义真核表达载体

采用脂质体法将其转染至SGC-7901胃癌细胞中

从光镜、电镜水平观察转染与未转染细胞形态学的变化。结果与亲本细胞相比

光镜下反义tankyrase 1基因转染的SGC-7901细胞胞体增大

胞浆丰富

核浆比例降低

核分裂象减少

核型变规则;透射电镜下核异型性减少

核膜较规则

细胞器丰富

部分细胞胞质内有微腺腔形成。结论反义Tankyrase 1基因转染能部分逆转SGC-7901细胞的恶性表型

有促进其分化的作用。

Abstract

Objective To study the effects of the transfection of antisense human tankyrase 1 on the morphology of SGC-7901.Method Antisense tankyrase 1 eukaryotic expression on vector pcDNA3.1 was constructed with recombinant DNA technique and transfected into SGC-7901 with liposome DOTAP.Morphological changes of the transfected and non-transfected cells were examined under light and electron microscopy.Results Under light microscopy

transfected cells with antisense gene to tankyrase 1 were larger with abundant cytoplasm and had less nuclei division.The ratio of nuclei to cytoplasm decreased as compared with their parent cells.Under electron microscopy

organelles in these cells were abundant and nuclear membrane was more regular.Conclusion Tankyrase 1 antisense gene can partially reverse malignant phenotypes of SGC-7901 cells and promote its differentiation.

关键词

Tankyrase 1真核表达载体反义基因胃癌

Keywords

Tankyrase 1Eukaryotic Expression VectorAntisense GeneGastric Cancer

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