Objective To identify the role of important mitosis gene CENP-E in aneuploidy formation and tumorigenesis

the expression vectors of CENP-E for RNA interference as pCENP-Es were designed and contructed.Method The interfering sequences of CENP-E were designed according to the sequence of CENP-E in GenBank.Four pairs of oligonucleotides were synthesized and inserted into plasmid pgenesil-1 to generate siRNA expression vector

and were identified by sequence analysis.The recombinant plasmid pCENP-Es and CENP-E expression plasmid pDSRED-Q2-Q3 was cotransfected into the cultured 293T cell line.The interfering eficiency was detected by RT-PCR.Results Four CENP-E siRNA frames were successfully inserted into the plasmid vector pgenesil-1

and the recombinant plasmids were sent to the sequence analysis.The result of RT-PCR indicates that the siRNA expression vector 1 and 4 got the best interfering eficiency as 84.47%.Conclusion The siRNA recombinant can be constructed successfully by RNAi technique for inhibiting CENP-E expression.