Role of microRNA-126 on regulation of VEGFmediated angiogenesis in vitro

任梦宇; 迟路湘; 欧书林

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Role of microRNA-126 on regulation of VEGFmediated angiogenesis in vitro

更新时间：2026-01-09

- Role of microRNA-126 on regulation of VEGFmediated angiogenesis in vitro
- Journal of Army Medical University Vol. 38, Issue 20, Pages: 2259-2263(2016)
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  CLC： R322.12;R329.28;R394.2
- Published：2016
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任梦宇,迟路湘,欧书林.microRNA-126对VEGF介导血管生成的调控作用[J].陆军军医大学学报(原第三军医大学学报),2016,38(20):2259-2263.

　Ren Mengyu,Chi Luxiang,Ou Shulin.Role of microRNA-126 on regulation of VEGFmediated angiogenesis in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(20):2259-2263.
任梦宇,迟路湘,欧书林.microRNA-126对VEGF介导血管生成的调控作用[J].陆军军医大学学报(原第三军医大学学报),2016,38(20):2259-2263. DOI：

　Ren Mengyu,Chi Luxiang,Ou Shulin.Role of microRNA-126 on regulation of VEGFmediated angiogenesis in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(20):2259-2263. DOI：

摘要

目的研究microRNA-126（miR-126）对血管内皮细胞生长发育的影响，探寻miR-126可能参与的血管生成过程。方法常规培养脐静脉内皮细胞（human umbilical vein endothelial cells

HUVECs）。构建miR-126过表达载体并转染HUVECs；实验分为：正常细胞组，空载慢病毒转染组，miR-126过表达慢病毒转染组（n=6）；于转染第6天后用qRT-PCR检测内皮生长负性调节因子spred1、Pik3R2的mRNA表达水平，Western blot检测spred1、Pik3R2的蛋白表达水平。结果转染第6天后，对qRT-PCR及Western blot检测结果进行分析，过表达组中spread1、Pik3R2的2（-ΔΔCt）值较其余两组低；过表达组中spred1/GAPDH和Pik3R2/GAPDH值也均小于其余两组，差异具有统计学意义（P0.05）。spred1、Pik3R2的mRNA表达水平明显下调，其蛋白表达水平也明显降低。结论 miR-126能促进VEGF的内皮细胞增殖及血管生成作用。

Abstract

Objective To investigate the role of microRNA-126 (miR-126) on regulation of vascular endothelial growth factor (VEGF) mediated angiogenesis. Methods Human umbilical vein endothelial cells (HUVECs) were cultured and transfected with lentiviral vectors carrying miR-126. The cells were divided into control group，empty vector group and miR-126 transfected group (LV3-miR-126). Total RNAs were collected at day 6 after transfection. The mRNA expression of negative regulatory factors

sprouty related EVH1 domain containing 1 (spred1) and phosphoinositide-3kinase regulatory subunit 2 (Pik3R2)

were measured by quantitative realtime polymerase chain reaction （qRT-PCR）. The protein expression of spred1 and Pik3R2 was detected by Western blot. Results The qRT-PCR results showed that spred1 and Pik3R2 were significantly down-regulated in LV3-miR126 group compared with the control and empty vector groups (P0.05). Also

their protein expression levels were obviously decreased with statistical significance (P0.05). Conclusion miR-126 expression can promote proliferation of endothelial cells and VEGF-mediated angiogenesis.

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