Objective      To analyze the pathogenic distribution and antibiotic susceptibility of chronic suppurative otitis media (CSOM) in order to guide  rational drug application. Methods     Middle ear pus samples were collected from 555 hospitalized patients (571 ears)  with chronic suppurative otitis media in our department from January 2013 to March 2015 and cultured for bacteria and antibiotic susceptibility test.  Results     There were 379 strains (66.37%) of microbes isolated from 571 ears

including 334 ears of bacteria (88.13%) and 47 ears (12.40%) of fungi. The most frequently isolated pathogens were Coagulase-negative Staphylococcus (191 ears

42.51%)

Staphylococcus aureus (58 ears

17.37%)

and Pseudomonas aeruginosa (31 ears

9.28%). The antibiotic sensitivity was variable among the isolated bacterial strains. Conclusion      Coagulase-negative Staphylococcus

Staphylococcus aureus and Pseudomonas aeruginosa are the main pathogenic bacteria in chronic suppurative otitis media. The susceptibility test are beneficial for choosing appropriate antibiotics.