Effects of dietary fatty acids on expression of gene sterol-regulatory elementary binding protein-1c and occurrence of non-alcoholic fatty liver disease in rats

刘裕; 高燕翔; 冉莉; 张勇; 张乾勇; 糜漫天

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Effects of dietary fatty acids on expression of gene sterol-regulatory elementary binding protein-1c and occurrence of non-alcoholic fatty liver disease in rats

更新时间：2026-01-09

- Effects of dietary fatty acids on expression of gene sterol-regulatory elementary binding protein-1c and occurrence of non-alcoholic fatty liver disease in rats
- Journal of Army Medical University Vol. 38, Issue 18, Pages: 2002-2007(2016)
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  CLC： R151.3;R363.27;R575.5
- Published：2016
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刘裕,高燕翔,冉莉,张勇,张乾勇,糜漫天.不同膳食脂肪酸对大鼠肝脏SREBP-1c基因表达和非酒精性脂肪肝发生的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(18):2002-2007.

　Liu Yu,Gao Yanxiang,Ran Li,Zhang Yong,Zhang Qianyong,Mi Mantian.Effects of dietary fatty acids on expression of gene sterol-regulatory elementary binding protein-1c and occurrence of non-alcoholic fatty liver disease in rats[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(18):2002-2007.
刘裕,高燕翔,冉莉,张勇,张乾勇,糜漫天.不同膳食脂肪酸对大鼠肝脏SREBP-1c基因表达和非酒精性脂肪肝发生的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(18):2002-2007. DOI：

　Liu Yu,Gao Yanxiang,Ran Li,Zhang Yong,Zhang Qianyong,Mi Mantian.Effects of dietary fatty acids on expression of gene sterol-regulatory elementary binding protein-1c and occurrence of non-alcoholic fatty liver disease in rats[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(18):2002-2007. DOI：

摘要

目的探讨不同膳食脂肪酸构成对非酒精性脂肪肝（nonalcoholic fatty liver disease

NAFLD）发生、发展的影响及其与固醇调节元件结合蛋白-1c（SREBP-1c）的关系。方法 100只成年SD大鼠，按随机数字表法分为5组：对照组、NAFLD模型组、单不饱和脂肪酸组（monounsaturated fatty acid

MUFA）、n-6多不饱和脂肪酸组（n-6 polyunsaturated fatty acids，n-6 PUFA）和4∶1 n-6/n-3 PUFA组，每组20只。对照组用基础饲料喂养，其余各组饲料中加1%胆固醇和10%不同油脂（分别是10%猪油、10%橄榄油、10%玉米油、8%玉米油+2%鱼油）。分别于第8、16周时，采用HE染色观察肝脏脂肪变性情况，测定血清和肝脏中脂质含量，采用实时定量PCR分析SREBP-1c、FAS mRNA表达，Western blot检测SREBP-1c、FAS的蛋白表达。结果 NAFLD模型组大鼠体质量显著高于对照组和其他膳食脂肪酸组（P0.05），模型组、MUFA组大鼠肝脏中TG含量显著高于对照组（P0.05），而n-6 PUFA组和4∶1 n-6/n-3 PUFA组大鼠血清中TG及肝脏中TC、TG浓度显著低于模型组（P0.05）；HE染色后，NAS量化评分显示模型组大鼠肝脏脂肪变性程度较对照组、MUFA组、n-6 PUFA组、4∶1 n-6/n-3 PUFA组严重；与对照组比较，NAFLD模型组大鼠肝脏SREBP-1c与FAS mRNA表达升高

MUFA组、n-6 PUFA组和4∶1 n-6/n-3 PUFA组SREBP-1c蛋白表达降低（P0.05）。结论降低膳食中饱和脂肪酸，增加不饱和脂肪酸（尤其是适当比例的n-6/n-3 PUFA）可下调高脂喂养大鼠肝内SREBP-1c的表达，延缓高脂膳食引起的NAFLD发生、发展。

Abstract

Objective To investigate the effects of dietary fatty acids on the development and progression of non-alcoholic fatty liver disease (NAFLD) and the correlation with the expression of sterol regulatory element binding protein-1c (SREBP-1c). Methods A total of 100 SD rats were randomly divided into 5 groups with different diets: control group (standard-chow diet)

NAFLD model group (standard-chow diet with 10% lard and 1% cholesterol)

mono-unsaturated fatty acid (MUFA) group(standard-chow diet with 10% olive oil and 1% cholesterol)

n-6 polyunsaturated fatty acid (n-6 PUFA) group (standard-chow diet with 10% corn oil and 1% cholesterol)

and 4∶1 n-6/n-3 poly-unsaturated fatty acid (4∶1 n-6/n-3 PUFA) group (standard-chow diet with 8% corn oil

2% fish oil and 1% cholesterol). Each group had 20 rats. The hepatic steatosis was observed by hematoxylin and eosin (HE) staining after feeding for 8 and 16 weeks. The concentrations of total cholesterol (TC) and triglyceride (TG) in serum and liver were measured. The mRNA levels of SREBP-1c and FAS were measured by quantitative real-time PCR

and their protein levels by Western blotting. Results In NAFLD model group

the body mass of rats were significantly higher than that in control group and other groups (P0.05). The TG level in the liver was significantly higher in NAFLD model group and MUFA group than that in control group (P0.05)

but TG levels in the serum

and TC and TG levels in the liver in both n-6 PUFA group and 4∶1 n-6/n-3PUFA group were significantly lower than those in NAFLD model group (P0.05). HE staining showed that the fatty degeneration in NAFLD model group was more severe than in control group

MUFA group

n-6 PUFA group and 4∶1 n-6/n-3 PUFA group. Compared to the control group

the expression levels of SREBP-1c and FAS were significantly higher in NAFLD model group

and the protein expression of SREBP-1c was significantly lower in MUFA group

n-6 PUFA group and 4∶1 n-6/n-3 PUFA group. Conclusion Increasing the amount of n-3 PUFA

particularly in a 4:1 ratio of n-6 PUFA to n-3 PUFA

and decreasing saturated fatty acid may prevent the occurrence and development of NAFLD in high-fat fed rats

which is partly modulated by down-regulation of SREBP-1c.

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