Objective      To determine the effect of stable miR200c over-expression on the cancer biological features of the oligometastatic cells in vitro.  MethodsAn oligometastatic cell line over-expressing miR200c was established by lentivirus infection (MDA-435-OL-miR200c). At the same time

a negative control cell line was generated by transfection with empty plasmid LV3NC. MDA-435-POL line

with confirmed polymetastatic phenotype in vivo served as a positive control for this study. The biological effect of miR200c over-expression was evaluated by CCK-8 assay for cell proliferation

agar colony formation assay for clonogeneicity

transwell migration assay and matrigel invasion assay for the invasiveness.  Results      Over-expression of miR200c resulted in the increases in the clonogenicity (cloning efficiency: 0.69±0.02)

cell migration (migrated cells number: 21.20±2.35) and invasion ability (invasive cells number: 56.80±5.22)

in the oligometastatic MDA-435-OL cells

and all the parameters were significantly higher than those of negative control (P0.05) and were similar to those of positive control. However

the miR200c over-expression had no effect on cell proliferation.   Conclusion      Stable over-expression of miR200c results in increases in clonogenicity

cell migration and invasion ability in the oligometastatic MDA-435-OL cells

which are resemble to those of MDA-435-POL polymetastatic cells.