GeneChip array for screening ATF3 related genes associated with acute lung injury in mouse model

钱斓兰; 郭亮; 吴学玲

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GeneChip array for screening ATF3 related genes associated with acute lung injury in mouse model

更新时间：2026-01-09

- GeneChip array for screening ATF3 related genes associated with acute lung injury in mouse model
- Journal of Army Medical University Vol. 38, Issue 24, Pages: 2571-2575(2016)
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  CLC： R394.3;R446.9;R563
- Published：2016
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钱斓兰,郭亮,吴学玲.基因芯片筛选急性肺损伤小鼠模型肺组织中活化转录因子3相关差异基因[J].陆军军医大学学报(原第三军医大学学报),2016,38(24):2571-2575.

　Qian Lanlan,Guo Liang,Wu Xueling.GeneChip array for screening ATF3 related genes associated with acute lung injury in mouse model[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(24):2571-2575.
钱斓兰,郭亮,吴学玲.基因芯片筛选急性肺损伤小鼠模型肺组织中活化转录因子3相关差异基因[J].陆军军医大学学报(原第三军医大学学报),2016,38(24):2571-2575. DOI：

　Qian Lanlan,Guo Liang,Wu Xueling.GeneChip array for screening ATF3 related genes associated with acute lung injury in mouse model[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(24):2571-2575. DOI：

摘要

目的利用基因芯片技术筛选LPS诱导的急性肺损伤小鼠肺组织中与活化转录因子3（acting -transcription factor 3，ATF3）相关的差异基因表达，以发现与ATF3可能相互作用的基因。方法采用基因芯片技术筛选野生型C57小鼠和ATF3敲基因小鼠经LPS处理后肺组织中的差异表达基因。进一步采用RT-PCR技术对重点基因的表达进行验证。结果与野生型C57小鼠比较，ATF3敲基因小鼠共筛选出1 692个差异基因，有21个与炎症调控相关的基因表达差异明显，其中，Myo18a、Mest、Gpr124、Rcan2、Tnfsf15、Ltbp3、Cdh2、Ppm1f、Spns2、Rarres2、Cgn、Mmrn2、Crb3、Adamts2、Mrc2、Sh3tc2、Cdk5rap1、Cenpf共18个基因在ATF3敲基因小鼠肺组织中表达明显上调；Padi4、Wfdc82个基因表达明显下调。对重点基因Tnfsf15（又称为TL1A）进一步验证，RT-PCR检测结果显示，表达趋势与基因芯片一致。结论 TL1A可能参与了ATF3对LPS诱导的急性肺损伤的炎症保护作用。

Abstract

Objective To screen the differentially expressed genes related to acting transcription factor 3 (ATF3) in the lung tissue of mouse model of lipopolysaccharides (LPS) induced acute lung injury (ALI) with GeneChip assay. Methods RNAs were isolated from lung tissues of wild-type (WT) mice and ATF3 knockout (KO) mice after LPS treatment. Differentially expressed genes were screened out by GeneChip array. Key genes were selected for further validation by real time (RT)-PCR. Results Analysis of genomic screen data showed that a total of 1 692 genes were identified differentially expressed in ATF3 KO mice compared with WT mice. Among them

21 genes were significantly-related to inflammatory regulation

including 18 up-regulated genes Myo18a

Mest

Gpr124

Rcan2

Tnfsf15

Ltbp3

Cdh2

Ppm1f

Spns2

Rarres2

Cgn

Mmrn2

Crb3

Adamts2

Mrc2

Sh3tc2

Cdk5rap1and Cenpf

and 2 down-regulated genes Padi4 and Wfdc8.The up-regulation of Tnfsf15 (also named TL1A) was verified by RT-PCR showing elevated mRNA expression. Conclusion TL1A maybe involved in the ATF3 mediated antiinflammation of LPS-induced ALI.

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Department of Geriatrics and Special Service Medicine， First Affiliated Hospital（Southwest Hospital）， Army Medical University （Third Military Medical University）

Institute of Field Surgery， Daping Hospital， Army Medical University

Laboratory of Stem Cell and Tissue Engineering， Chongqing Medical University

Department of Human Anatomy， Anhui Medical University

Department of Anesthesiology, Department of War Wound Shock and Transfusion， Army Medical Center of PLA/Daping Hospital of Third Military Medical University

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