Objective      To investigate the infection status and genotype distribution of human papillomavirus (HPV) in Chongqing women in order to provide theoretical basis for the development of preventive vaccine and its epidemiological study.       Methods      Cervical exfoliative cells from 32 882 Chongqing woman were collected to detect HPV genotyping with Cape flow-through hybridization. Then the results were analyzed with infection characteristics of the patients.       Results      The overall HPV positive rate was 19.20%

in which 81.12% were infected by high-risk types. The rate of single infection accounted for 80.52%

and that of double infection for 15.18%. There were totally 21 HPV subtypes detected in the study. The detection rates of high-risk HPV subtypes were relatively high

and were 18.90%

16.67% and 12.99% respectively for HPV subtypes 52

16 and 58. The relatively high detection rates for low-risk HPV subtype were CP8304 (7.94%) and subtype 6 (3.60%). The HPV subtypes were distributed differently at different ages. The infection rates of high risk HPV were 22.22%

15.29%

14.52%

15.29%

19.50%

27.36% and 38.36%

respectively for the patients aged no more than 20 years old group

21 to 30 years old group

31 to 40 years old group

41 to 50 years old group

51 to 60 years old group

61 to 70 years old group

and older than 71 years old group

with significant differences among them (Chi square=25.754

P0.05).       Conclusion      Cervical HPV infection are mainly high-risk HPV subtypes and single type infection in the females of Chongqing. The leading genotypes of HPV subtypes are 52

16 and 58.