Effect of curcumin on sensitivity of breast cancer cells to cisplatin

邹娇; 陈彬; 李强; 朱琳琳; 付晓红; 王祥卫

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Effect of curcumin on sensitivity of breast cancer cells to cisplatin

更新时间：2026-01-09

- Effect of curcumin on sensitivity of breast cancer cells to cisplatin
- Journal of Army Medical University Vol. 38, Issue 16, Pages: 1809-1814(2016)
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  CLC： R282.71;R737.9;R979.19
- Published：2016
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邹娇,陈彬,李强,朱琳琳,付晓红,王祥卫.姜黄素对乳腺癌细胞顺铂敏感性的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(16):1809-1814.

　Zou Jiao,Chen Bin,Li Qiang,Zhu Linlin,Fu Xiaohong,Wang Xiangwei.Effect of curcumin on sensitivity of breast cancer cells to cisplatin[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(16):1809-1814.
邹娇,陈彬,李强,朱琳琳,付晓红,王祥卫.姜黄素对乳腺癌细胞顺铂敏感性的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(16):1809-1814. DOI：

　Zou Jiao,Chen Bin,Li Qiang,Zhu Linlin,Fu Xiaohong,Wang Xiangwei.Effect of curcumin on sensitivity of breast cancer cells to cisplatin[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(16):1809-1814. DOI：

摘要

目的探讨姜黄素（curcumin）对乳腺癌细胞中顺铂（Cisplatin，CDDP）治疗敏感性的影响及其可能机制。方法 CCK8检测、Hoechst染色观察CDDP、姜黄素单独及联合用药48 h对MCF7细胞增殖抑制和细胞凋亡的影响；Western blot分析MCF7细胞在CDDP（0、1.25、2.5、5、10、20 μg/mL）、姜黄素（0、1、5、20、30、50、100 μmol/L）单独及联合处理后FEN1(flap endonuclease 1)表达水平的变化；CCK8检测沉默FEN1对MCF7细胞中CDDP敏感性的影响。结果 CDDP、姜黄素均可以剂量依赖方式抑制MCF7细胞增殖，其IC50分别为5、30 μmol/L；与单独2 μg/mL CDDP处理组比较，2 μg/mL CDDP联合20 μmol/L姜黄素、30 μmol/L姜黄素处理组对细胞增殖的抑制效应明显增强[分别为（84.1±0.8）%、（51.1±0.5）%、（29.4±0.3）%，P0.05]；与单独20 μmol/L姜黄素处理组比较，20 μmol/L 姜黄素联合2 μg/mL CDDP、5 μg/mL CDDP处理组对细胞增殖的抑制效应也明显增强[分别为（76.9±0.7）%、（42.4±0.3）%、(31.6±0.4)%，P0.05]；2 μg/mL CDDP联合20 μmol/L姜黄素组的细胞凋亡明显增强（P0.05）；与Control siRNA组比较，FEN1 siRNA+CDDP组可显著增强CDDP抑制MCF7细胞增殖的敏感性[（25.4±0.3）% vs（18.7±0.2）%，P0.05]；CDDP增殖抑制无效浓度或低浓度时，FEN1蛋白的表达随CDDP的处理剂量依赖性上调，而姜黄素可剂量依赖性下调FEN1蛋白表达；与单独CDDP和姜黄素处理组比较，2 μg/mL CDDP联合20 μmol/L姜黄素组可显著降低FEN1蛋白表达（P0.05）。结论姜黄素可增强CDDP对乳腺癌细胞的敏感性，其机制与降低细胞FEN1的表达有关。

Abstract

Objective To explore the effect of curcumin on the sensitivity of breast cancer cells to cisplatin (CDDP) and possible mechanism. Methods CCK8 assay and Hoechst staining were used to observe the proliferation and apoptosis of MCF7 cells treated with curcumin alone or in combination with CDDP for 48 h. Western blotting was used to detect the expression level of flap endonuclease 1 (FEN1) in MCF7 cells treated as above. The sensitivity of FEN1silenced MCF7 cells to CDDP was detected by CCK8 assay. Results Both CDDP and curcumin could inhibit MCF7 cell proliferation in a dosedependent manner

and the IC50 values of CDDP and curcumin were 5 and 30 μmol/L

respectively. Compared with the cells treated with 2 μg/mL CDDP alone

the proliferation of the cells treated with CDDP in combination with 20 or 30 μmol/L curcumin was significantly inhibited [(84.1±0.8)% vs (51.1±0.5)%

(84.1±08)% vs(29.4±0.3)%

respectively

P0.05]. Compared with the cells treated with curcumin alone

the proliferation of the cells treated with 20 μmol/L curcumin in combination with 2 or 5 μg/mL CDDP was also significantly inhibited [(76.9±0.7)% vs (542.4±0.3)%

(76.9±0.7)% vs(31.6±0.4)%

respectively

P0.05]. Apoptosis of the cells treated with 2 μg/mL CDDP in combination with 20 μmol/L curcumin was obviously increased (P0.05). Compared with control treatment

silencing FEN1 with siRNA could enhance the sensitivity of MCF7 cell to CDDP (P0.05). FEN1 protein was upregulated in a dosedependent manner in the cells treated with low concentration or invalid concentration of CDDP. However

curcumin could obviously downregulate FEN1 protein in a dosedependent manner. Compared with the cells treated with CDDP or curcumin alone

FEN1 protein expression was significantly reduced in the cells treated with 2 μg/mL CDDP in combination with 20 μmol/L curcumin (P0.05). Conclusion Curcumin can enhance the sensitivity of breast cancer cells to CDDP

which is related with the decrease of FEN1 expression.

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