Objective      To establish a method to stereoselectively synthesize cerebrosterol.       Methods       The target molecule was synthesized through 9 steps including methyl esterification

reduction

Wittig reaction

  Sharpless catalytic asymmetric dihydroxylation

etc.

with hyodeoxycholic acid as starting material

demosterol acetate as key intermediate

and  Sharpless catalytic asymmetric dihydroxylation as key step.       Results      Cerebrosterol was synthesized with the total yield of 52% and stereoselectivity of 97% de

and it was identified by mass spectrometry (MS)

proton magnetic resonance spectroscopy (

1

HNMR)

carbon magnetic resonance spectroscopy (

13

CNMR)

infrared spectroscopy (IR)

elemental analysis

etc.       Conclusion      The synthesis of cerebrosterol is chara

cterized by high yield

high stereoselctivity

easy recrystallization and purification of each intermediate

and feasible scalability.