Correlation of high-risk human papillomavirus infection and diversity of vaginal microbiota

潘颖; 盛华芳; 康玲; 马啸; 郑慧敏; 周宏伟; 刘木彪

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Correlation of high-risk human papillomavirus infection and diversity of vaginal microbiota

更新时间：2026-01-09

- Correlation of high-risk human papillomavirus infection and diversity of vaginal microbiota
- Journal of Army Medical University Vol. 38, Issue 13, Pages: 1559-1564(2016)
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  CLC： R322.65;R372;R373.9
- Published：2016
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潘颖,盛华芳,康玲,马啸,郑慧敏,周宏伟,刘木彪.高危型人乳头状瘤病毒感染与阴道菌群的相关性研究[J].陆军军医大学学报(原第三军医大学学报),2016,38(13):1559-1564.

　Pan Ying,Sheng Huafang,Kang Ling,Ma Xiao,Zheng Huimin,Zhou Hongwei,Liu Mubiao.Correlation of high-risk human papillomavirus infection and diversity of vaginal microbiota[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(13):1559-1564.
潘颖,盛华芳,康玲,马啸,郑慧敏,周宏伟,刘木彪.高危型人乳头状瘤病毒感染与阴道菌群的相关性研究[J].陆军军医大学学报(原第三军医大学学报),2016,38(13):1559-1564. DOI：

　Pan Ying,Sheng Huafang,Kang Ling,Ma Xiao,Zheng Huimin,Zhou Hongwei,Liu Mubiao.Correlation of high-risk human papillomavirus infection and diversity of vaginal microbiota[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(13):1559-1564. DOI：

摘要

目的探索高危型人乳头瘤状病毒（high-risk human papillomavirus

HR-HPV）感染阴性与阳性女性之间阴道菌群构成的差异。方法采集2014年9月至2015年9月间于我科就诊的33例未感染HR-HPV（HPV

）女性及98例感染HR-HPV（HPV

）患者的阴道分泌物，分别对每个样本进行细菌总基因组DNA提取、 16S rRNA V4区基因扩增及采用Illumina 高通量测序技术对扩增的PCR产物进行测序等步骤，然后通过BIPES、TSC、GAST等程序，分析阴道菌群物种丰度和结构，并对两组女性的阴道菌群构成进行比较。结果 HPV

组女性阴道菌群以乳酸杆菌为优势菌属，占81.54%，同时含有少量的加德纳菌属、链球菌属、普氏菌属等。HPV

组患者阴道菌群中乳酸杆菌属则显著减少（40.48%），加德纳菌属显著增加（32.35%），同时普氏菌属、奇异菌属、厌氧球菌属等多种厌氧菌属相对丰度也有所增加。在HPV

组女性的阴道菌群中发现布鲁菌属的存在，而HPV

组中未见此菌属。结论阴道菌群失调与HR-HPV感染之间存在相关性

HR-HPV感染者阴道菌群中乳酸杆菌属显著减少，加德纳菌属等多种厌氧菌属过度增殖，并发现有布鲁菌属存在。

Abstract

Objective To explore the diversities of the vaginal microbiota in high-risk human papillomavirus (HR-HPV )-positive (HPV

) and HR-HPV-negative (HPV

) women from the point of view of bacterial community structure. Methods A total of 131 vaginal swabs were collected from 33 HPV

women and 98 HPV

patients who visited in our hospital between September 2014 and September 2015. The total DNA of microbes from

the samples were isolated for amplifying the 16S rRNA V4 hypervariable region by PCR and pyrosequencing by Illumina. BIPES

TSC and GAST were used to analyze the information of the vaginal microbiota from the samples. Results Lactobacillus was the predominant genus in HPV

－

women (81.54%)

with a small quantity of Gardnerella

Streptococcus

Prevotella and other genus. The amount of Lactobacillus was decreased (40.48%) and that of Gardnerella increased (32.35%) significantly in the HPV

women. Moreover

the relative abundances of Prevotella

Atopobium

and Anaerococcus were increased in different degrees. Brucella was the taxon only found in HPV

women but not in HPV

－

women. Conclusion Vaginal dysbacteriosis is associated with HR-HPV infection. The HR-HPV infected patients have Lactobacillus decreased significantly

Gardnerella and a variety of anaerobic bacteria proliferated excessively

and Brucella existence.

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