Resveratrol protects hepatocytes against hypoxia/reoxygenation injury and its relationship with TLR4/NF-κB pathway

何雕; 张国庆; 郑道峰; 魏续福; 刘锐; 吴忠均

您当前的位置：

首页 >

文章列表页 >

Resveratrol protects hepatocytes against hypoxia/reoxygenation injury and its relationship with TLR4/NF-κB pathway

更新时间：2026-01-09

- Resveratrol protects hepatocytes against hypoxia/reoxygenation injury and its relationship with TLR4/NF-κB pathway
- Journal of Army Medical University Vol. 38, Issue 12, Pages: 1398-1403(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5;R322.47;R364.4
- Published：2016
- 稿件说明：
移动端阅览
何雕,张国庆,郑道峰,魏续福,刘锐,吴忠均.白藜芦醇在缺氧/复氧诱导的肝细胞损伤中的保护作用及与TLR4/NF-κB通路的关系[J].陆军军医大学学报(原第三军医大学学报),2016,38(12):1398-1403.

　He Diao,Zhang Guoqing,Zheng Daofeng,Wei Xufu,Liu Rui,Wu Zhongjun.Resveratrol protects hepatocytes against hypoxia/reoxygenation injury and its relationship with TLR4/NF-κB pathway[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(12):1398-1403.
何雕,张国庆,郑道峰,魏续福,刘锐,吴忠均.白藜芦醇在缺氧/复氧诱导的肝细胞损伤中的保护作用及与TLR4/NF-κB通路的关系[J].陆军军医大学学报(原第三军医大学学报),2016,38(12):1398-1403. DOI：

　He Diao,Zhang Guoqing,Zheng Daofeng,Wei Xufu,Liu Rui,Wu Zhongjun.Resveratrol protects hepatocytes against hypoxia/reoxygenation injury and its relationship with TLR4/NF-κB pathway[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(12):1398-1403. DOI：

摘要

目的探讨白藜芦醇（resveratrol，RES）在缺氧/复氧（hypoxia reoxygenation，H/R）诱导的大鼠肝细胞损伤中的保护作用及其相关分子机制。方法建立BRL-3A细胞（大鼠肝细胞株）H/R模型。建模前分别用RES和TLR4抑制剂（HTA125）预处理细胞。建模完成后

检测细胞存活率及细胞凋亡，观察细胞形态变化，检测细胞培养液中谷丙转氨酶（alanine transaminase

ALT)及炎症因子含量，检测细胞TLR4、NF-κB p65基因mRNA及蛋白水平表达及NF-κB p65入核情况。结果 H/R条件使细胞存活率明显降低，细胞凋亡率增加（P0.01），受损细胞明显增多，ALT、IL-1β含量增多（P0.01），TLR4和NF-κB p65表达水平显著提高（P0.01），p65入核细胞比例提高（P0.01）。经RES及HTA125预处理后，细胞存活率显著提高，细胞凋亡比例显著缩小（P0.01），受损细胞减少，ALT、IL-1β含量降低（P0.01），TLR4和NF-κB p65表达水平明显降低（P0.01），p65入核细胞比例下降（P0.01）。结论 RES可以减轻H/R诱导的BRL-3A肝细胞损伤，该作用可能与抑制TLR4/NF-κB信号通路有关。

Abstract

Objective To determine the protective effect of resveratrol (RES) on hepatocytes induced by hypoxia/reoxygenation (H/R) and investigate the molecular mechanism. Methods Before H/R model was established

BRL-3A cells were treated with RES or HTA125 (TLR4 inhibitor). Survival rate and apoptosis were detected in the cells. Alanine transaminase (ALT) and interleukin-1β (IL-1β) in the culture were determined. The mRNA and protein expression levels of TLR4 and NF-κB p65 in the cells were determined by quantitative real-time PCR and Western blotting. Results H/R stimulation decreased cell survival rate and enhanced the apoptosis (P0.01). The expression levels of ALT and IL-1β were enhanced significantly (P0.01)

and the expression levels of TLR4 and NF-κB p65 were markedly increased (P0.01). After pretreatment with RES or HTA125

the cell survival rate was increased (P0.01) while the apoptosis decreased (P0.01). ALT and IL-1β were reduced significantly (P0.01)

and the expression levels of TLR4 and NF-κB p65 were decreased (P0.01). Moreover

RES inhibited the translocation of NF-κB p65 after H/R stimulation in BRL-3A cells (P0.01). Conclusion RES can alleviate the hepatocyte injury induced by H/R

which may be related with inhibition of TLR4/NF-κB signaling pathway.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Hypoxia/reoxygenation attenuates migration and invasion of human extracellular villus trophoblast cells via DNA methyltransferase pathway

Resveratrol promotes hepatic lipid decomposition by regulating ATGL/CGI58 and Rab7/LC3β through perilipin 2

Resveratrol improves renal inflammatory injury in exhaustive exercise rats by regulating NOD-like receptor protein 3 inflammasome

Resveratrol promotes axonal regeneration via PI3K/Akt pathway in rats after intracerebral hemorrhage

Resveratrol protects human umbilical vein endothelial cells against oxidative injury by activating transcription factor EB in vitro

Related Author

胡司晨

兰曦

丁裕斌

李方方

陈恩祥

付利娟

杨依林

张艳

Related Institution

No data

⁰