Protective effects of agmatine on oxidative stress induced by LPS in RAW264.7 cells

侯凤艳; 罗莉; 朱俊宇; 康文渊; 范霞; 柴鉴深; 卢中秋; 梁华平

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Protective effects of agmatine on oxidative stress induced by LPS in RAW264.7 cells

更新时间：2026-01-09

- Protective effects of agmatine on oxidative stress induced by LPS in RAW264.7 cells
- Journal of Army Medical University Vol. 38, Issue 9, Pages: 949-953(2016)
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  CLC： R392.12;R966;R977.4
- Published：2016
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侯凤艳,罗莉,朱俊宇,康文渊,范霞,柴鉴深,卢中秋,梁华平.胍丁胺对脂多糖诱导RAW264.7细胞氧化应激损伤的保护作用[J].陆军军医大学学报(原第三军医大学学报),2016,38(09):949-953.

　Hou Fengyan,Luo Li,Zhu Junyu,Kang Wenyuan,Fan Xia,Chai Jianshen,Lu Zhongqiu,Liang Huaping.Protective effects of agmatine on oxidative stress induced by LPS in RAW264.7 cells[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(09):949-953.
侯凤艳,罗莉,朱俊宇,康文渊,范霞,柴鉴深,卢中秋,梁华平.胍丁胺对脂多糖诱导RAW264.7细胞氧化应激损伤的保护作用[J].陆军军医大学学报(原第三军医大学学报),2016,38(09):949-953. DOI：

　Hou Fengyan,Luo Li,Zhu Junyu,Kang Wenyuan,Fan Xia,Chai Jianshen,Lu Zhongqiu,Liang Huaping.Protective effects of agmatine on oxidative stress induced by LPS in RAW264.7 cells[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(09):949-953. DOI：

摘要

目的探讨胍丁胺（agmatine

AGM）对脂多糖（lipopolysaccharides，LPS）诱导小鼠巨噬细胞系RAW264.7氧化应激的抑制作用及其机制。方法 RAW264.7细胞经LPS（10 μg/mL）刺激发生氧化应激，同时AGM（1 mmol/L）对其进行干预。分为对照组、LPS组、LPS+AGM组、AGM组。药物作用24 h，以2

7-二氢二氯荧光素二乙酸酯(2′

7′-dichlorofluoresce in diacetate

DCFH-DA)为荧光探针检测细胞内活性氧（reactive oxygen species，ROS）水平；Griess法检测细胞内一氧化氮（NO）水平；Western blot检测细胞内的iNOS蛋白及细胞质细胞核内Nrf2蛋白表达；RT-PCR检测Nrf2下游抗氧化酶血红素氧合酶(heme oxygenase-1

HO-1) mRNA的表达。结果与对照组相比，LPS可显著增加RAW264.7细胞中ROS、NO以及iNOS蛋白水平，而AGM干预后上述指标含量均明显降低（P0.05）。AGM干预组中细胞核的Nrf2表达及其下游分子HO-1 mRNA表达水平较LPS单独刺激组显著升高，表明Nrf2信号通路在AGM的作用下被活化。结论 AGM可通过活化转录因子Nrf2促进抗氧化酶HO-1的表达，进而减轻细胞内的氧化应激损伤。

Abstract

Objective To determine the inhibitory effect of agmatine (AGM) on lipopolysaccharides (LPS)-induced oxidative stress in mouse macrophage RAW264.7 cells and its underlying mechanism. Methods RAW264.7 cells were stimulated by LPS (10 μg/mL) to induce oxidative stress

and treated with 1 mmol/L AGM. Thus the cells were divided into control

LPA

LPS+AGM

and AGM groups. In 24 h after the treatment

reactive oxygen species (ROS) level of these cells was determined with 2

7-dichlorofluoresce in diacetate (DCFH-DA) as fluorescence probe

intracellular NO level by Greiss method

intracellular iNOS protein and cytoplasmic/nuclear Nrf2 protein expression by Western blotting

and mRNA expression of heme oxygenase-1 (HO-1) by RT-PCR. Results Compared with the control group

LPS significantly induced the levels of ROS

NO and iNOS protein in RAW264.7 cells

and AGM significantly reversed such effect (P0.05). Nrf2 pathway and its target HO-1 were significantly up-regulated compared to LPS-stimulated cells

suggesting that AGM activated the Nrf2 signaling pathway. Conclusion AGM reduces oxidative damage of the RAW264.7 cells by promoting HO-1 antioxidase expression through activation of the Nrf2 pathway.

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