Optical fiber-based recording of Ca<sup>2+</sup> activity dynamics of entorhinal-hippocampal projections in mice

卢建; 金文均; 覃涵; 谌小维

您当前的位置：

首页 >

文章列表页 >

Optical fiber-based recording of Ca²⁺ activity dynamics of entorhinal-hippocampal projections in mice

更新时间：2026-01-09

- Optical fiber-based recording of Ca²⁺ activity dynamics of entorhinal-hippocampal projections in mice
- Journal of Army Medical University Vol. 38, Issue 11, Pages: 1229-1234(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R319;R329.25;R338.26
- Published：2016
- 稿件说明：
移动端阅览
卢建,金文均,覃涵,谌小维.内嗅皮层-海马投射末梢基于光纤技术的钙活动记录[J].陆军军医大学学报(原第三军医大学学报),2016,38(11):1229-1234.

　Lu Jian,Jin Wenjun,Qin Han,Chen Xiaowei.Optical fiber-based recording of Ca2+ activity dynamics of entorhinal-hippocampal projections in mice[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(11):1229-1234.
卢建,金文均,覃涵,谌小维.内嗅皮层-海马投射末梢基于光纤技术的钙活动记录[J].陆军军医大学学报(原第三军医大学学报),2016,38(11):1229-1234. DOI：

　Lu Jian,Jin Wenjun,Qin Han,Chen Xiaowei.Optical fiber-based recording of Ca2+ activity dynamics of entorhinal-hippocampal projections in mice[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(11):1229-1234. DOI：

摘要

目的运用光纤探测系统建立轴突末梢Ca

活动的光学记录方法，并初步探究麻醉和自由活动状态下小鼠皮层-海马通路的活动规律。方法向8～10周龄雄性C57BL/6小鼠内侧内嗅皮层（medial entorhinal cortex，MEC）浅层（Ⅱ层）注射神经元特异并携带Ca

荧光指示蛋白基因的腺相关病毒（AAV-Syn-GCaMP5G），继续饲养1个月后将光纤植入到海马齿状回（dentate gyrus

DG）分子层上沿，记录麻醉和自由活动状态下轴突末梢的Ca

活动；相同方法记录AAV-Syn-增强绿色荧光蛋白（eGFP）小鼠作为对照。向MEC浅层注射河豚毒素（tetrodotoxin

TTX），同步记录轴突末梢Ca

活动，注射人工脑脊液（artificial cerebrospinal fluid

ACSF）作为对照。结果内嗅皮层Ⅱ层神经元投射至海马DG的轴突纤维密集表达GCaMP5G。在麻醉和自由活动的小鼠中均能稳定记录到轴突末梢Ca

信号，eGFP对照小鼠未记录到类似Ca

信号。MEC浅层注射TTX后，轴突末梢Ca

信号100 s内最高幅度降至注射前的12.6%。与ACSF比较，TTX对轴突末梢Ca

活动有明显阻断作用（P0.01）。内嗅皮层投射至DG的轴突末梢处Ca

活动在麻醉状态下以一定节律同步发放，自由活动状态下则表现为无节律非同步发放，且CaCa

信号幅度有所增强。结论通过GCaMP5G特异标记了内侧内嗅皮层Ⅱ层投射至DG的轴突纤维，并运用光纤探测系统建立了长远投射轴突末梢Ca

活动的光学记录方法。与麻醉状态相比，自由活动状态下内嗅-海马通路呈现更强的Ca

活动发放。

Abstract

Objective To establish a method of recording Ca

activity at axonal terminals of neural projection and then study the activity pattern of the pathway from the entorhinal cortex t

o the hippocampus of mice under anesthetized and freely behaving conditions. Methods AAV-Syn-GCaMP5G was injected into the medial entorhinal cortex (MEC) superficial layer of male C57BL/6 mice at age of 8~10 weeks. In 1 month later

optical fiber was implanted onto the stratum moleculare of dentate gyrus (DG) to record Ca

activity at the axonal terminals under both anesthetized and freely behaving conditions. The AAV-Syn-eGFP injected mice were recorded as the control group. Ca

activity dynamics at the axon terminal sites were compared before and after tetrodotoxin (TTX) was injected into the MEC superficial layers of the anesthetized mice. The mice in the control group were injected with artificial cerebrospinal fluid (ACSF). Results The axonal fibers projecting from the entorhinal cortex to DG were densely labeled by GCaMP5G. Ca

signals from the axonal terminals can be recorded steadily under both anesthetized and freely behaving conditions by using optical fiber photometry. However

no signals were detected in eGFP-labeled mice. After injecting TTX into the superficial layers of MEC

the maximum amplitude of Ca

signals within 100 s from axonal terminals descended to 12.6% of Ca

signals recorded before injection. Compared with the ACSF-injected mice

TTX significantly decreased Ca

activity at the axon terminals (P0.01). The Ca

activity from the axonal terminals in the hippocampus exhibited unsynchronized pattern and higher amplitude in freely behaving condition as compared with the rhythmic-synchronized pattern under anesthetized condition. Conclusion Utilizing specific labeling of neuronal projection with GCaMP5G and optical fiber photometry

signals from the axonal terminal sites of long-range projections can be recorded reliably under both anesthetized and freely behaving conditions. The Ca

activity from the axonal terminals of entorhinal-hippocampal projec

tions may exhibit higher level of dynamics in freely behaving mice.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of clesmatine on myelination and neuronal activity during sleep in the hippocampus of adult APP/PS1 mice

Ovarian estrogen deficiency participates in the pathogenesis of Alzheimer’s disease by regulating hippocampal mitochondrial function and extracellular matrix remodeling

Effect of sleep deprivation on firing activity of hippocampal parvalbumin-expressing interneurons associated with trace eyeblink conditioning

Changes in dentate gyrus neuronal activity during sleepwake cycle in mice

Genetic deletion of GluN3A affects depression-like behaviors and mRNA transcriptome in medial prefrontal cortex and hippocampus in mice

Related Author

YANG Ye

ZHANG Haibo

LIU Yanji

YAO Zhongxiang

HU Bo

ZHANG Xuan

ZHOU Maohu

MENG Zhaoyou

Related Institution

Experimental Center of Basic Medicine, College of Basic Medical Sciences， Army Medical University （Third Military Medical University）

Department of Physiology, College of Basic Medical Sciences， Army Medical University （Third Military Medical University）

Department of Neurobiology， College of Basic Medical Sciences， Army Medical University （Third Military Medical University）

Sports Medicine Center， First Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Neurology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

⁰

Optical fiber-based recording of Ca2+ activity dynamics of entorhinal-hippocampal projections in mice

DOI：

摘要

Abstract

关键词

Keywords

references

Optical fiber-based recording of Ca²⁺ activity dynamics of entorhinal-hippocampal projections in mice