Effect of pVAX1-IL-4/SYN-B DNA vaccine on expression of neurotrophic factors in MPTP-induced chronic Parkinson’s disease mice

陈亚兰; 雷朋; 张玉平; 楼金核; 鲁凤娇; 贾桢; 彭国光

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Effect of pVAX1-IL-4/SYN-B DNA vaccine on expression of neurotrophic factors in MPTP-induced chronic Parkinson’s disease mice

更新时间：2026-01-09

- Effect of pVAX1-IL-4/SYN-B DNA vaccine on expression of neurotrophic factors in MPTP-induced chronic Parkinson’s disease mice
- Journal of Army Medical University Vol. 37, Issue 24, Pages: 3417-2421(2015)
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  CLC： R341;R392.7;R742.5
- Published：2015
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陈亚兰,雷朋,张玉平,楼金核,鲁凤娇,贾桢,彭国光.pVAX1-IL-4/SYN-B核酸疫苗对帕金森病模型小鼠纹状体神经营养因子表达的影响[J].陆军军医大学学报(原第三军医大学学报),2015,37(24):3417-2421.

　Chen Yalan,Lei Peng,Zhang Yuping,Lou Jinhe,Lu Fengjiao,Jia Zhen,Peng Guoguang.Effect of pVAX1-IL-4/SYN-B DNA vaccine on expression of neurotrophic factors in MPTP-induced chronic Parkinson’s disease mice[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(24):3417-2421.
陈亚兰,雷朋,张玉平,楼金核,鲁凤娇,贾桢,彭国光.pVAX1-IL-4/SYN-B核酸疫苗对帕金森病模型小鼠纹状体神经营养因子表达的影响[J].陆军军医大学学报(原第三军医大学学报),2015,37(24):3417-2421. DOI：

　Chen Yalan,Lei Peng,Zhang Yuping,Lou Jinhe,Lu Fengjiao,Jia Zhen,Peng Guoguang.Effect of pVAX1-IL-4/SYN-B DNA vaccine on expression of neurotrophic factors in MPTP-induced chronic Parkinson’s disease mice[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(24):3417-2421. DOI：

摘要

目的探索预防注射pVAX1-IL-4/SYN-B核酸疫苗对1-甲基-4-苯基-1

6-四氢吡啶 (1-methyl-4-phenyl-1

6-tetrahydropyridine

MPTP)诱导的慢性帕金森病模型小鼠纹状体神经营养因子表达的影响，进一步探讨核酸疫苗神经保护作用的具体机制。方法 48只C57BL/6雄性小鼠完全随机分为4组：正常对照组、空质粒组、PBS组和核酸疫苗组，每组12只；分别肌注PBS、空质粒、PBS和pVAX1-IL-4/SYN-B核酸疫苗，然后给予后3 组小鼠注射MPTP 建立慢性帕金森病模型。采用自由活动实验观察小鼠行为学变化；Western blot、免疫组化检测纹状体区酪氨酸羟化酶（tyrosine hydroxylase，TH）表达；Western blot检测小鼠纹状体胶质细胞源性神经营养因子（glial cell line-derived neurotrophic factor，GDNF）、脑源性神经营养因子（brain derived neurotrophic factor，BDNF）表达。结果与PBS 组和空质粒组比较，核酸疫苗组小鼠运动迟缓症状明显改善（P0.05），纹状体区GDNF、BDNF表达升高（P0.05），TH丢失明显减少（P0.05）。结论 pVAX1-IL-4/SYN-B核酸疫苗预防免疫可增加慢性MPTP帕金森病模型小鼠纹状体区神经营养因子表达，具有一定的神经保护作用。

Abstract

Objective To evaluate the effect of pVAX1-IL-4/SYN-B DNA vaccines on the expression of neurotrophic factors in the striatum of mice with chronic Parkinson’s disease (PD) induced with 1-methyl-4-phenyl-1

6-tetrahydropyridine (MPTP). Methods C57BL/6 mice were randomly divided into normal control group

blank plasmid group

PBS group

and DNA vaccine group (n=12 for each group). The anterior tibial muscles of the mice hind leg were injected with PBS

empty plasmid

PBS

and pVAX1-IL4/SYN-B

respectively. Then the chronic PD mice were built by intraperitoneal injection of MPTP

while the normal control group was injected with the same volume of PBS. The behavior changes of the mice were observed. The expression of tyrosine hydroxylase (TH) in the striatum of mice was detected by Western blotting and immunohistochemistry

and the expression of glial cell-derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF) in the striatum was detected by Western blotting. Results Compared with the PBS group or blank plasmid group

the DNA vaccine group showed improved motor symptoms (P0.05)

reduced TH loss (P0.05)

and increased expression of GDNF and BDNF (P0.05). Conclusion The pVAX1-IL-4/SYN-B DNA vaccine shows neuroprotective effect in MPTP-induced chronic PD mice by enhancing the expression of neurotrophic factors GDNF and BDNF in the striatum.

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