Resveratrol attenuates Aβ<sub>25-35 </sub>induced neurotoxicity via activation of SIRT1/autophagy signaling pathway

邓皓月; 糜漫天

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Resveratrol attenuates Aβ_25-35induced neurotoxicity via activation of SIRT1/autophagy signaling pathway

更新时间：2026-01-09

- Resveratrol attenuates Aβ_25-35induced neurotoxicity via activation of SIRT1/autophagy signaling pathway
- Journal of Army Medical University Vol. 38, Issue 6, Pages: 619-622(2016)
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  CLC： R151.3;R322.81;R741.05
- Published：2016
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邓皓月,糜漫天.白藜芦醇通过上调SIRT1/自噬通路改善Aβ25-35对PC12细胞增殖的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2016,38(06):619-622.

　Deng Haoyue,Mi Mantian.Resveratrol attenuates Aβ25-35 induced neurotoxicity via activation of SIRT1/autophagy signaling pathway[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(06):619-622.
邓皓月,糜漫天.白藜芦醇通过上调SIRT1/自噬通路改善Aβ25-35对PC12细胞增殖的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2016,38(06):619-622. DOI：

　Deng Haoyue,Mi Mantian.Resveratrol attenuates Aβ25-35 induced neurotoxicity via activation of SIRT1/autophagy signaling pathway[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(06):619-622. DOI：

摘要

目的探讨SIRT1/自噬通路在白藜芦醇改善Aβ

25-35

诱导的PC12细胞增殖活力下降中的作用。方法用不同浓度（1、5、10、20 μmol/L）白藜芦醇 (resveratrol

RSV) 单独或加入5 mmol/L自噬特异抑制剂（3-methyladenine

3-MA）、2 μmol/L SIRT1选择性抑制剂（EX527）处理PC12细胞 2 h后，再加入30 μmol/L淀粉样蛋白25-35片段（Amyloid β-protein fragment 25-35

Aβ

25-35

）处理24 h。采用CCK-8检测细胞增殖活力，电镜检测自噬体，Western blot检测自噬标志蛋白P62、LC3和SIRT1蛋白的表达水平。结果 30 μmol/L Aβ

25-35

可显著降低PC12细胞的增殖活力，为对照组的45.14%（P0.05）。RSV预处理可显著改善Aβ

25-35

诱导的PC12细胞增殖活力下降（P0.05），且随浓度增加，保护作用增强。同时，RSV可明显促进自噬体的形成，上调SIRT1、LC3-Ⅱ的表达和降低P62的表达，且随浓度增加，表达变化越显著；而加入3-MA和EX527处理能显著抑制RSV的上述效应。结论 RSV可通过激活SIRT1/自噬通路改善Aβ

25-35

诱导的神经细胞毒性。

Abstract

Objective To determine whether resveratrol (RSV) attenuates Aβ

25-35

induced neurotoxicity through SIRT1/autophagy signaling pathway. Methods PC12 cells were treated with different concentrations (1

10 and 20 μmol/L) of RSV for 2 h in the presence or absence of 5 mmol/L 3-methyladenine (3-MA

specific inhibitor of autophagy ) or 2 μmol/L EX527 (selective inhi

bitor of SIRT1)

following by treatment of 30 μmol/L amyloid β-protein fragment 25-35 (Aβ

25-35

) for 24 h. CCK-8 assay was used to detect the cell viability

and electron microscope was applied to detect autophagosome. The expressions of P62

LC3 and SIRT1 were detected by Western blotting. Results Aβ

25-35

(30 μmol/L) remarkably decreased the viability of PC12 cells

which was 45.14% of the control group P0.05). Pretreatment with RSV significantly improved Aβ

25-35

induced decrease of cell viability in a dose-dependent manner (P0.05). Simultaneously

RSV promoted the formation of autophagosome

up-regulated the expressions of SIRT1 and LC3-Ⅱ and down-regulated the expression of P62 in a dose-dependent manner. However 3-MA and EX527 abolished the above effects of RSV. Conclusion RSV attenuates Aβ

25-35

induced neurotoxicity through activation of SIRT1/autophagy signaling pathway.

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Resveratrol attenuates Aβ25-35 induced neurotoxicity via activation of SIRT1/autophagy signaling pathway

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摘要

Abstract

关键词

Keywords

references

Resveratrol attenuates Aβ_25-35induced neurotoxicity via activation of SIRT1/autophagy signaling pathway