Effect of miR-378 on proliferation and apoptosis in myelodysplastic syndrome cells

匡兴怡; 魏春梅; 张涛; 王利

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Effect of miR-378 on proliferation and apoptosis in myelodysplastic syndrome cells

更新时间：2026-01-09

- Effect of miR-378 on proliferation and apoptosis in myelodysplastic syndrome cells
- Journal of Army Medical University Vol. 38, Issue 6, Pages: 604-608(2016)
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  CLC： R394-33;R394.2;R551.302
- Published：2016
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匡兴怡,魏春梅,张涛,王利.miR-378对骨髓增生异常综合征细胞凋亡及增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(06):604-608.

　Kuang Xingyi,Wei Chunmei,Zhang Tao,Wang Li.Effect of miR-378 on proliferation and apoptosis in myelodysplastic syndrome cells[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(06):604-608.
匡兴怡,魏春梅,张涛,王利.miR-378对骨髓增生异常综合征细胞凋亡及增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(06):604-608. DOI：

　Kuang Xingyi,Wei Chunmei,Zhang Tao,Wang Li.Effect of miR-378 on proliferation and apoptosis in myelodysplastic syndrome cells[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(06):604-608. DOI：

摘要

目的研究miR-378对骨髓增生异常综合征SKM-1细胞凋亡和增殖的影响。方法我们分别用miR-378重组慢病毒和阴性对照病毒感染SKM-1细胞。然后采用CCK-8检测miR-378对SKM-1细胞生长的影响，流式细胞仪检测各实验组细胞凋亡和周期情况，并用Western blot检测凋亡过程中的关键因子cleaved-caspase-3蛋白的表达。用生物信息学方法预测miR-378可能的靶基因，用PCR和Western blot进行初步验证。结果 miR-378慢病毒转染组的细胞增殖活性明显降低，流式细胞术检测miR-378组的细胞凋亡率为（12.90±3.72）%，明显高于阴性对照病毒转染组和空白对照组 [（3.21±1.91）%、（2.78±1.04）%，P0.01]；miR-378组的G0/G1期细胞增多，S期细胞减少。同时，Western blot结果显示miR-378过表达引起了cleaved-caspase-3的表达升高。用生物信息学方法预测抗凋亡蛋白基因BCL2L2为miR-378潜在的靶基因，并发现miR-378可以抑制BCL2L2蛋白的表达。结论 miR-378可以通过引起细胞凋亡和细胞周期阻滞来抑制SKM-1细胞的增殖，并降低BCL2L2蛋白的表达。

Abstract

Objective To investigate the effect of miR-378 on apoptosis and proliferation in myelodysplastic syndrome SKM-1 cells. Methods SKM-1 cells were divided into 3 groups: SKM-1 cells transfected with lentiviral vector over-expressing miR-378 (miR-378 group)

SKM-1 cells transfected with empty lentiviral vector (negative control group) and normal SKM-1 (blank control group). CCK-8 assay and Annexin V/7-AAD staining were conducted to detect the effect of miR-378 on the proliferation and apoptosis of SKM-1 cells. Then

Western blotting was performed to detect the expression of cleaved-capase-3 protein. Bioinformatics analysis was conducted to predict the potential target gene of miR-378

and quantitative reverse transcriptase-polymerase chain reaction and Western blotting were further confirm the target gene. Results After transfection with lentiviral vector over-expressing miR-378

the growth of SKM-1 cells in the miR-378 group was inhibited obviously. The apoptotic rate [(12.90 ± 3.72)%] in the miR-378 group was significantly higher than [(3.21 ± 1.91)%] in the negative control group and [(2.78 ± 1.04)%] in the blank control group (P0.01). The percentage of SKM-1 cells in G0/G1 phase was increased and the percentage in S phase was decreased. Western blotting showed that overexpression of miR-378 resulted in up-regulation of cleaved-caspase-3. Moreover

BCL2L2 gene was predicted as potential target gene of miR-378

and overexpression of miR-378 reduced the expression of BCL2L2 protein. Conclusion miR-378 can inhibit proliferation of SKM-1 cells by inducing apoptosis and cell cycle arrest

and it also down-regulates the expression of BCL2L2 protein.

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