Objective      To determine the expression levels of hypoxia-inducible factor 1α (HIF-1α) and glutaminase 2 (GLS2) in colorectal cancer and explore their clinical significance.       Methods      Immunohistochemical staining was applied for the detection of HIF-1α and GLS2 in 108 samples of colorectal cancer and 23 adjacent mucosa tissues obtained through surgical treatment. The correlation between the expression of HIF-1α and GLS2 and clinicopathological features of patients with colorectal cancer was analyzed. Spearman rank correlation was used to detect the correlation between HIF-1α expression and GLS2 expression，Kaplan-Meier survival analysis was used to evaluate the impact of HIF-1α and GLS2 expression on postoperative survival of the patients with colorectal cancer. Cox univariate and multivariate prognostic analysis was utilized to explore the prognostic factors of the patients with colorectal cancer.       Results      The expression levels of HIF-1α and GLS2 were both significantly higher than in adjacent mucosa tissues (P0.01). HIF-1α expression was closely correlated with age

TNM stage

lymphatic metastasis and postoperative distant metastasis (P0.05)

and GLS2 expression was closely correlated with age

invasion depth and postoperative distant metastasis (P0.05). Spearman rank correlation analysis indicated that HIF-1α and GLS2 expression were positively correlated with the prognosis of colorectal cancer (r=0.464

P0.01). Multivariate Cox analysis revealed that TNM stage

postoperative distant metastasis

and expression of HIF-1α and GLS2 played significant roles in the prediction of patient’s prognosis (P0.05).       Conclusion      High expression of HIF-1α and GLS2 predicts poor prognosis in the patients with colorectal cancer

indicating that they might play important roles in the occurrence and progression of the disease.