Expression and immunological evaluation of BsaL, type Ⅲ secretion system protein of Burkholderia pseudomallei

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Expression and immunological evaluation of BsaL, type Ⅲ secretion system protein of Burkholderia pseudomallei

更新时间：2026-01-09

- Expression and immunological evaluation of BsaL, type Ⅲ secretion system protein of Burkholderia pseudomallei
- Journal of Army Medical University Vol. 38, Issue 6, Pages: 585-588(2016)
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  CLC： R341;R378.99;R394-33
- Published：2016
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方瑶,胡艺,朱攀,任春艳,马腾飞,王昆,毛旭虎.类鼻疽伯克霍尔德菌Ⅲ型分泌系统蛋白BsaL的制备和免疫反应性分析[J].陆军军医大学学报(原第三军医大学学报),2016,38(06):585-588.

　Fang Yao,Hu Yi,Zhu Pan,Ren Chunyan,Ma Tengfei,Wang Kun,Mao Xuhu.Expression and immunological evaluation of BsaL, type Ⅲ secretion system protein of Burkholderia pseudomallei[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(06):585-588.
方瑶,胡艺,朱攀,任春艳,马腾飞,王昆,毛旭虎.类鼻疽伯克霍尔德菌Ⅲ型分泌系统蛋白BsaL的制备和免疫反应性分析[J].陆军军医大学学报(原第三军医大学学报),2016,38(06):585-588. DOI：

　Fang Yao,Hu Yi,Zhu Pan,Ren Chunyan,Ma Tengfei,Wang Kun,Mao Xuhu.Expression and immunological evaluation of BsaL, type Ⅲ secretion system protein of Burkholderia pseudomallei[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(06):585-588. DOI：

摘要

目的构建表达重组的类鼻疽伯克霍尔德菌Ⅲ型分泌系统结构蛋白BsaL的大肠工程菌，制备高纯度、高质量的重组BsaL蛋白样品，探讨其作为类鼻疽菌检测的候选抗原的可行性。方法基因工程技术构建表达重组BsaL蛋白的大肠工程菌，通过亲和层析、凝胶过滤层析等纯化方法得到高纯度的BsaL蛋白。包被BsaL蛋白于96孔板，分析其用于检测临床类鼻疽菌株的敏感性和特异性。结果成功构建了表达重组BsaL蛋白的大肠工程菌，诱导、表达及纯化后得到高纯度(99%)、高浓度（20 mg/mL）的重组BsaL蛋白。该蛋白在类鼻疽菌检测方面表现出较好的灵敏度（85%）和特异性（89%）。结论成功表达了重组的BsaL蛋白，获得了高纯度的BsaL蛋白样品，在类鼻疽菌的诊断方面展示出较好的应用价值。

Abstract

Objective To express recombinant BsaL

type Ⅲ secretion system protein of Burkholderia pseudomallei

for detecting the bacterium based on enzyme linked immunosorbent assay (ELISA). Methods PET-28a/BsaL plasmid was constructed and introduced into Escherichia coli BL21. BsaL-His was induced by IPTG and purified by affinity chromatography and gel filtration chromatography. Then

the recombinant protein was applied to 96-well plate for detecting Burkholderia pseudomallei-containing samples. Results BsaL-His with high purity (99%) and high concentration (20 mg/mL) was prepared and applied to detecting Burkholderia pseudomallei at relative high specificity (89%) and sensitivity (85%). Conclusion BsaL protein is successfully expressed and purified

and it can be applied to detecting Burkholderia pseudomallei.

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