Effect of Jumonji domain-containing protein 2B on biological behaviors in human cutaneous squamous cell carcinoma A431 cells

王月; 余南岚; 翟志芳; 钟白玉; 熊亚; 张恋; 冯林; 杨希川

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Effect of Jumonji domain-containing protein 2B on biological behaviors in human cutaneous squamous cell carcinoma A431 cells

更新时间：2026-01-09

- Effect of Jumonji domain-containing protein 2B on biological behaviors in human cutaneous squamous cell carcinoma A431 cells
- Journal of Army Medical University Vol. 38, Issue 7, Pages: 697-701(2016)
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  CLC： R345;R73-362;R739.5
- Published：2016
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王月,余南岚,翟志芳,钟白玉,熊亚,张恋,冯林,杨希川.JMJD2B对人皮肤鳞状细胞癌A431细胞株生物学行为的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(07):697-701.

　Wang Yue,Yu Nanlan,Zhai Zhifang,Zhong Baiyu,Xiong Ya,Zhang Lian,Feng Lin,Yang Xichuan.Effect of Jumonji domain-containing protein 2B on biological behaviors in human cutaneous squamous cell carcinoma A431 cells[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(07):697-701.
王月,余南岚,翟志芳,钟白玉,熊亚,张恋,冯林,杨希川.JMJD2B对人皮肤鳞状细胞癌A431细胞株生物学行为的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(07):697-701. DOI：

　Wang Yue,Yu Nanlan,Zhai Zhifang,Zhong Baiyu,Xiong Ya,Zhang Lian,Feng Lin,Yang Xichuan.Effect of Jumonji domain-containing protein 2B on biological behaviors in human cutaneous squamous cell carcinoma A431 cells[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(07):697-701. DOI：

摘要

目的探讨组蛋白去甲基化酶 JMJD2B(Jumonji domain-containing protein 2B)小干扰RNA(siRNA)对人皮肤鳞状细胞癌A431细胞株生物学行为的影响。方法采用免疫组化和细胞免疫荧光的方法检测JMJD2B在人皮肤鳞状细胞癌组织和A431细胞中的表达，转染JMJD2B siRNA至A431细胞株，分别采用 CCK-8、克隆形成实验、流式细胞仪、Transwell法检测细胞增殖、克隆、周期分布、凋亡、迁移及侵袭情况。结果 JMJD2B在肿瘤组织中的表达高于正常皮肤。与未转染组和转染对照组比较，JMJD2B siRNA转染组增殖、克隆、迁移及侵袭能力显著受抑，肿瘤细胞发生G1期阻滞，细胞凋亡比例增加（P0.05）。结论 JMJD2B在人皮肤鳞状细胞癌中呈高表达，JMJD2B siRNA能促进A431细胞的凋亡，同时也抑制了肿瘤细胞的增殖、克隆、迁移以及侵袭的能力。

Abstract

Objective To investigate the inhibitory effect of Jumonji domain-containing protein 2B (JMJD2B) siRNA on biological behaviors in human squamous carcinoma cells. Methods The expression of JMJD2B in the human squamous cell carcinoma tissue and A431 cells was detected by immunohistochemical staining and cell immunofluorescence assay. The A431 cells were transfected with JMJD2B siRNA by Liposome. Then the proliferation

colony formation

cell apoptosis

cell cycle

migration and invasion were measured by CCK-8 assay

colony formation assay

flow cytometry

and transwell migration assay

respectively.

Results JMJD2B was highly expressed in the tumor tissues than normal skin tissues. Compared with the un-transfected A431 cells

the cell proliferation

colony formation

and migration and invasion abilities were significantly decreased

while cell apoptosis was increased in the cells after siRNA transfection (P0.05). Cell cytometry indicated JMJD2B siRNA transfection arrested the cells at G

phase (P0.05). Conclusion JMJD2B is highly expressed in the human squamous cell carcinoma. JMJD2B siRNA promotes cell apoptosis

and suppresses cell proliferation

colony formation

and migration and invasion abilities in A431 cells.

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