Gsdermin A3 suppresses Krt6b expression in mouse skin keratinocytes

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Gsdermin A3 suppresses Krt6b expression in mouse skin keratinocytes

更新时间：2026-01-09

- Gsdermin A3 suppresses Krt6b expression in mouse skin keratinocytes
- Journal of Army Medical University Vol. 38, Issue 3, Pages: 238-244(2016)
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  CLC： R322.991;R329.25;R394.2
- Published：2016
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刘颖新,白秀峰,赖向东,郭海英,罗燕,余裕,连小华.Gsdermin A3基因在小鼠皮肤角质形成细胞中对Krt6b基因表达的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(03):238-244.

　Liu Yingxin,Bai Xiufeng,Lai Xiangdong,Guo Haiying,Luo Yan,Yu Yu,Lian Xiaohua.Gsdermin A3 suppresses Krt6b expression in mouse skin keratinocytes[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(03):238-244.
刘颖新,白秀峰,赖向东,郭海英,罗燕,余裕,连小华.Gsdermin A3基因在小鼠皮肤角质形成细胞中对Krt6b基因表达的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(03):238-244. DOI：

　Liu Yingxin,Bai Xiufeng,Lai Xiangdong,Guo Haiying,Luo Yan,Yu Yu,Lian Xiaohua.Gsdermin A3 suppresses Krt6b expression in mouse skin keratinocytes[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(03):238-244. DOI：

摘要

目的初步探讨Gsdermin A3（Gsdma3）基因对Krt6b基因表达的影响。方法通过组织切片的免疫荧光观察、RT-PCR、Q-PCR、Western blot等方法检测毛囊生长期[出生后8 d（P8d）]、毛囊退化期（P16d）、毛囊静止期（P25d）的Gsdma3基因突变鼠（AE鼠）和野生型鼠（WT鼠）背部皮肤Krt6b基因的表达情况。利用免疫荧光进一步观察注射有Gsdma3和RFP腺病毒的Gsdma3基因突变鼠和野生型鼠的Krt6b基因的表达情况。结果 Gsdma3基因突变鼠的Krt6b基因表达量在各个时相点均高于野生型鼠(P0.05)。其中，在出生后16 d Krt6b基因表达量达到最高，而在出生后25 d降至最低。对Gsdma3基因突变鼠进行基因回补后，免疫荧光、实时定量PCR及Western blot检测结果显示Krt6b基因表达减弱。同时，对野生型鼠过表达Gsdma3基因后，免疫荧光、实时定量PCR及Western blot结果显示Krt6b基因表达量降低。结论小鼠皮肤角质形成细胞中，Krt6b基因的表达受Gsdma3基因抑制。

Abstract

Objective To determine the effect of Gsdermin A3 (Gsdma3) on the expression of Krt6b. Methods Q-PCR

RT-PCR

Western blotting and immunohistochemical staining were used to detect the expression of Krt6b at mRNA and protein levels in Gsdma3 mutant mice (AE) and C57BL/6J wild-type mice (WT) at 3 time points in mouse hair follicle cycle (anagen: 8 d postnatally

catagen: 16 d postnatally

telogen: 25 d postnatally). Immunofluorescence assay was also used to determine the effect of the adenoviruses carrying Gsdma3 gene (AdGsdma3) or control gene (AdRFP) on the expression of Krt6b in AE and WT mice. Results The expression level of Krt6b was obviously higher in Gsdma3 mutant mice than WT mice (P0.05). At P16

the expression of Krt6b reached the highest level

while on P25 it reduced to the lowest level. Immunofluorescence assay

real-time PCR and Western blotting showed that the expression of Krt6b was decreased in Gsdma3 mutant mice after Gsdma3 expression rescue. Meanwhile

immunofluorescence assay showed that the expression of Krt6b was also decreased in WT mice with Gsdma3 over-expression. Conclusion Gsdma3 inhibits the expression of Krt6b in mouse keratinocytes.

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