Knockdown of scinderin attenuates self-renewal capacity in gastric cancer stem cells

刘佳佳; 刘军言; 严鹏; 吴益西; 姬成东; 王艳霞; 向东方; 钱锋

您当前的位置：

首页 >

文章列表页 >

Knockdown of scinderin attenuates self-renewal capacity in gastric cancer stem cells

更新时间：2026-01-09

- Knockdown of scinderin attenuates self-renewal capacity in gastric cancer stem cells
- Journal of Army Medical University Vol. 37, Issue 23, Pages: 2324-2328(2015)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R341;R394.2;R735.2
- Published：2015
- 稿件说明：
移动端阅览
刘佳佳,刘军言,严鹏,吴益西,姬成东,王艳霞,向东方,钱锋.沉默肌切蛋白表达抑制胃癌干细胞自我更新能力[J].陆军军医大学学报(原第三军医大学学报),2015,37(23):2324-2328.

　Liu Jiajia,Liu Junyan,Yan Peng,Wu Yixi,Ji Chengdong,Wang Yingxia,Xiang Dongfang,Qian Feng.Knockdown of scinderin attenuates self-renewal capacity in gastric cancer stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(23):2324-2328.
刘佳佳,刘军言,严鹏,吴益西,姬成东,王艳霞,向东方,钱锋.沉默肌切蛋白表达抑制胃癌干细胞自我更新能力[J].陆军军医大学学报(原第三军医大学学报),2015,37(23):2324-2328. DOI：

　Liu Jiajia,Liu Junyan,Yan Peng,Wu Yixi,Ji Chengdong,Wang Yingxia,Xiang Dongfang,Qian Feng.Knockdown of scinderin attenuates self-renewal capacity in gastric cancer stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(23):2324-2328. DOI：

摘要

目的探讨肌切蛋白（scinderin，SCIN）在调控胃癌干细胞自我更新能力中的作用。方法采用shRNA慢病毒干扰技术构建沉默SCIN表达的MGC803胃癌细胞系和XN0422原代胃癌细胞，同时构建各自的阴性对照(mock)细胞；以平板克隆形成和成球实验分别检测沉默SCIN对胃癌细胞克隆形成和成球能力的影响；裸鼠皮下移植瘤模型观察沉默SCIN对胃癌细胞体内成瘤能力的影响。结果 SCIN在胃癌干细胞中的表达显著高于普通贴壁细胞；沉默SCIN显著降低胃癌细胞的克隆和成球能力，MGC803细胞中 SCIN敲低细胞与mock细胞的克隆形成数分别为（26.33±4.37）和（62.33±3.18），肿瘤球形成数分别为（12.33±0.88）和（39.00±2.69），而在XN0422 细胞中，克隆形成数分别为（14.67±3.38）和（49.67±2.19），肿瘤球形成数分别为（15.67±1.76）和（33.33±1.65）（P0.01）；SCIN敲低的MGC803和 XN0422细胞较其mock细胞所形成的裸鼠皮下移植瘤，体积显著减小、质量显著减轻，差异有统计学意义（P0.01）。结论 SCIN基因沉默能有效抑制胃癌干细胞自我更新能力，可能成为针对胃癌肿瘤干细胞治疗的新靶点。

Abstract

Objective To determine the role of scinderin (SCIN) in the regulation of self-renewal capacity of gastric cancer stem cells. Methods SCIN-knockdown gastric cancer cells

including gastric cancer cell line MGC803 and primary gastric cancer XN0422 cells

were established with SCIN specific shRNA (shSCIN). Colony and tumorsphere formation assays were used to estimate the effect of shSCIN on self-renewal capability of gastric cancer cells in vitro

and the xenograft assay in nude mice was employed to assess the effect of shSCIN on tumorigenecity of gastric cancer cells. Results The expression of SCIN was markedly higher in gastric cancer stem cells than gastric cancer cells. SCIN knockdown significantly decreased the capabilities of colony and tumorsphere formation in MGC803 and XN0422 cells. The colony number derived from SCIN knockdown and mock cells was 26.33±4.37 and 62.33±3.18 in MGC803 cells

and 14.67±3.38 and 49.67±2.19 in XN0422 cells

respectively. The number of tumorsphere formatted by SCIN knockdown and mock cells was 12.33±0.88 and 39.00±2.69 in MGC803 cells

and 15.67±1.76 and 33.33±1.65 in XN0422 cells (P0.01 for all)

respectively. Xenograft assay showed that the volume and weight of the tumor mass derived from SCIN-knockdown MGC803 and XN0422 cells were remarkably smaller/lighter than that of mock cells (P0.05 for all). Conclusion Silencing of SCIN can effectively inhibit the self-renewal of gastric cancer stem cells

suggesting that SCIN might be a promising target for gastric cancer therapy by targeting gastric cancer stem cells.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Oxaliplatin suppresses gastric cancer by inducing cuproptosis

Effects of LncRNA FGD5-AS1 on migration and invasion of gastric cancer cells by regulating miR-302b-3p/E2F1 axis

Immunosuppressive microenvironment of TGIF1-overexpressing gastric cancers and its clinical significance

Diagnostic value of endoscopic LCPL sign for high-risk intestinal metaplasia in gastric mucosa

Effect of calumenin on metastasis and invasion of gastric cancer and prognosis of patients

Related Author

RAO Hang

YANG Hong

XIONG Mengyuan

ZHANG Liyong

HAN Xiao

DENG Boshao

WANG Lulu

CHEN Jian

Related Institution

Department of Immunology, College of Basic Medical Sciences， Army Medical University （Third Military Medical University）

Department of General Surgery， First Affiliated Hospital（Southwest Hospital）, College of Basic Medical Sciences， Army Medical University （Third Military Medical University）

Endoscopy Center, Qingdao Central Hospital of Rehabilitation University

Department of Gastroenterology, Qingdao Central Hospital of Rehabilitation University

Department of Gastroenterology， Chongqing Hechuan District People’s Hospital

⁰