Effect of Id1 gene on reversing malignant biological behaviors and inducing osteogenic differentiation of human osteosarcoma cells

仇超; 康权; 迭小红; 罗光金; 吕凤香; 肖程; 罗庆

您当前的位置：

首页 >

文章列表页 >

Effect of Id1 gene on reversing malignant biological behaviors and inducing osteogenic differentiation of human osteosarcoma cells

更新时间：2026-01-09

- Effect of Id1 gene on reversing malignant biological behaviors and inducing osteogenic differentiation of human osteosarcoma cells
- Journal of Army Medical University Vol. 38, Issue 4, Pages: 344-349(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R73-362;R730.23;R738.1
- Published：2016
- 稿件说明：
移动端阅览
仇超,康权,迭小红,罗光金,吕凤香,肖程,罗庆.Id1基因对人骨肉瘤细胞恶性逆转向成骨分化的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(04):344-349.

　Qiu Chao,Kang Quan,Die Xiaohong,Luo Guangjin,Lyu Fengxiang,Xiao Cheng,Luo Qing.Effect of Id1 gene on reversing malignant biological behaviors and inducing osteogenic differentiation of human osteosarcoma cells[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(04):344-349.
仇超,康权,迭小红,罗光金,吕凤香,肖程,罗庆.Id1基因对人骨肉瘤细胞恶性逆转向成骨分化的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(04):344-349. DOI：

　Qiu Chao,Kang Quan,Die Xiaohong,Luo Guangjin,Lyu Fengxiang,Xiao Cheng,Luo Qing.Effect of Id1 gene on reversing malignant biological behaviors and inducing osteogenic differentiation of human osteosarcoma cells[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(04):344-349. DOI：

摘要

目的探讨分化抑制因子1（inhibitor of differentiation factor 1

Id1）对人骨肉瘤细胞MG63恶性逆转向成骨分化的影响。方法用重组腺病毒调控MG63细胞中Id1过表达后，RT-PCR及Western blot检测验证Id1的表达情况，CCK-8检测细胞增殖能力，划痕实验检测细胞迁移能力，Transwell实验检测细胞侵袭能力，流式细胞术检测细胞周期，ALP读数法检测成骨分化早期指标ALP的活力。结果 AdId1重组腺病毒可以使MG63细胞中的Id1表达量增高，AdsiId1重组腺病毒可以使MG63细胞中的Id1表达量降低（P0.05）；Id1过表达后，MG63细胞的增殖能力、迁移能力、侵袭能力等恶性生物学行为均增强，G

期细胞比例降低，Id1表达量下调后则逆转MG63细胞的恶性生物学行为，G

期细胞比例增高（P0.05）；Id1表达量下调后，正常成骨分化的早期指标碱性磷酸酶（ALP）表达量增高（P0.05）。结论抑制Id1基因表达可以促使骨肉瘤的恶性生物学行为逆转并诱导骨肉瘤细胞发生成骨分化。

Abstract

Objective To investigate the effect of inhibitor of differentiation factor 1 (Id1) on reversing the malignant biological behaviors and inducing osteogenic differentiation of human osteosarcoma cells. Methods Human osteosarcoma cell line MG63 was infected with recombinant adenoviruses AdId1

AdsiId1 and AdGFP separately. RT-PCR and Western blot assay were used to determine the expression of Id1 in MG63 cells

and the cell proliferation

migration and invasion were detected by CCK-8 assay

scratch wound ass

and Transwell assay

respectively. Alkaline phosphatase (ALP) numeration was used to detect the early osteogenic potential of MG63 cells

and the cell cycle was detected by flow cytometry. Results The mRNA and protein expression of Id1 gene was increased after infection with AdId1 and decreased after infection with AdsiId1 (P0.05). CCK-8 assay

scratch wound assay and Transwell assay results showed that the malignant behaviors including cell proliferation

migration and invasion were significantly increased after AdId1 infection

and significantly decreased after AdsiId1 infection (P0.05). The percentage of G

-phase cells was decreased after infection with AdId1 and increased after infection with AdsiId1 (P0.05). AdsiId1 infection could enhance the activity of ALP in MG63 cells (P0.05). Conclusion Inhibiting the expression of Id1 gene can reverse the malignant biological behaviors and induce the osteogenic differentiation of human osteosarcoma cells.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression of miR-616 in osteosarcoma and its role in proliferation, apoptosis, migration and invasion of tumor cells

Application of 3D-printed customized prostheses in limb-salvage surgery for pediatric osteosarcoma of the distal femur: a case series study

Endothelial cells promote osteosarcoma cell metastasis by inhibiting LGI4 expression and activating NOTCH4 signaling pathway

Inhibitor of differentiation-1 promotes angiogenesis of human osteosarcoma by regulating VEGF-A

Related Author

仇超

康权

迭小红

罗光金

吕凤香

肖程

罗庆

Kang Quan

Related Institution

Department of Pediatric Oncology, Institute of Pediatrics, Key Laboratory of Child Development and Disorders of Ministry of Education, Chongqing Key Laboratory of Pediatrics,Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Children's Hospital of Chongqing Medical University

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Orthopedics， Affiliated Children’s Hospital， Chongqing Medical University

Third Department of Orthopaedics, Second Hospital, Lanzhou University

⁰