UTP attenuates migration of gastric cancer cells via PTEN/vimentin

万晗星; 谢睿; 刘菁菁; 杨仕明; 董辉

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UTP attenuates migration of gastric cancer cells via PTEN/vimentin

更新时间：2026-01-09

- UTP attenuates migration of gastric cancer cells via PTEN/vimentin
- Journal of Army Medical University Vol. 38, Issue 6, Pages: 550-553(2016)
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  CLC： R342.4;R730.23;R735.2
- Published：2016
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万晗星,谢睿,刘菁菁,杨仕明,董辉.尿苷三磷酸通过PTEN/Vimentin抑制胃癌细胞的侵袭[J].陆军军医大学学报(原第三军医大学学报),2016,38(06):550-553.

　Wan Hanxing,Xie Rei,Liu Jingjing,Yang Shiming,Dong Hui.UTP attenuates migration of gastric cancer cells via PTEN/vimentin[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(06):550-553.
万晗星,谢睿,刘菁菁,杨仕明,董辉.尿苷三磷酸通过PTEN/Vimentin抑制胃癌细胞的侵袭[J].陆军军医大学学报(原第三军医大学学报),2016,38(06):550-553. DOI：

　Wan Hanxing,Xie Rei,Liu Jingjing,Yang Shiming,Dong Hui.UTP attenuates migration of gastric cancer cells via PTEN/vimentin[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(06):550-553. DOI：

摘要

目的研究尿苷三磷酸（uridine triphosphate

UTP）对胃癌细胞迁移和侵袭的影响及其分子机制。方法采用细胞划痕实验检测UTP对胃癌细胞MKN-45和SGC-7901迁移能力的影响；Transwell检测UTP对正常人胃黏膜细胞GES-1和胃癌细胞MKN-45、SGC-7901侵袭能力的影响；Western blot检测UTP对胃癌细胞MKN-45和SGC-7901 PTEN磷酸化水平及Vimentin表达的影响。结果细胞划痕实验表明UTP组抑制胃癌细胞MKN-45和SGC-7901迁移（P0.05）。Transwell实验结果显示UTP组抑制胃癌细胞MKN-45和SGC-7901侵袭（P0.01），但对正常人胃黏膜细胞GES-1无影响(P0.05)。Western blot实验证实UTP组可增强胃癌细胞MKN-45和SGC-7901 PTEN磷酸化水平，同时下调Vimentin的表达。结论 UTP抑制胃癌细胞的迁移和侵袭，可能是与其上调PTEN磷酸化水平，抑制Vimentin表达有关。

Abstract

Objective To determine the effect of uridine triphosphate (UTP) on the migration and invasion of gastric cancer cells and investigate related molecular mechanism. Methods Wound scratch assay was applied for examining the migration of gastric cancer MKN-45 and SGC-7901 cells. Transwell migration assay was used to analyze the invasion of human gastric mucosal GES-1 cells

MKN-45 and SGC-7901 cells. Western blot analysis was carried out to detect the phosphorylation of PTEN and the expression of vimentin. Results UTP inhibited the migration and invasion of MKN-45 and SGC-7901 cells (P0.05)

but not of GES-1 cells (P0.05). Western blot analysis showed that UTP increased the phosphorylation level of PTEN in MKN-45 and SGC-7901 cells

and meanwhile

down-regulated the expression of vimentin. Conclusion UTP inhibits the migration and invasion of gastric cancer cells

probably through inducing the phosphorylation of PTEN and down-regulating the expression of vimentin.

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