Protein kinase CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole promotes apoptosis of human ovarian cancer CAOV3 cells

闫威; 任丽莉; 孟智超; 郭淼; 刘乙蒙; 刘超; 肖建英

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Protein kinase CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole promotes apoptosis of human ovarian cancer CAOV3 cells

更新时间：2026-01-09

- Protein kinase CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole promotes apoptosis of human ovarian cancer CAOV3 cells
- Journal of Army Medical University Vol. 37, Issue 14, Pages: 1478-1481(2015)
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  CLC： R73-361;R730.23;R737.31
- Published：2015
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闫威,任丽莉,孟智超,郭淼,刘乙蒙,刘超,肖建英.蛋白激酶CK2抑制剂四溴苯三唑可促进卵巢癌CAOV3细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2015,37(14):1478-1481.

　Yan Wei,Ren Lili,Meng Zhichao,Guo Miao,Liu Yimeng,Liu Chao,Xiao Jianying.Protein kinase CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole promotes apoptosis of human ovarian cancer CAOV3 cells[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(14):1478-1481.
闫威,任丽莉,孟智超,郭淼,刘乙蒙,刘超,肖建英.蛋白激酶CK2抑制剂四溴苯三唑可促进卵巢癌CAOV3细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2015,37(14):1478-1481. DOI：

　Yan Wei,Ren Lili,Meng Zhichao,Guo Miao,Liu Yimeng,Liu Chao,Xiao Jianying.Protein kinase CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole promotes apoptosis of human ovarian cancer CAOV3 cells[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(14):1478-1481. DOI：

摘要

目的观察蛋白激酶CK2抑制剂四溴苯三唑（4，5，6，7-tetrabromobenzotriazole，TBB）对卵巢癌CAOV3细胞增殖、凋亡及细胞凋亡调控因子Bcl-2、Bax表达的影响，进一步探讨TBB促进卵巢癌CAOV3细胞凋亡的作用机制。方法体外培养CAOV3细胞，将其分为对照组、DMSO组和TBB组（终浓度为12.5、25、50、75 μmol/L）。MTT测定各组细胞增殖情况；流式细胞仪检测各组卵巢癌CAOV3细胞凋亡；Western blot检测各组CAOV3细胞CK2α及凋亡相关因子Bcl-2、Bax的表达。结果与对照组[（92.00±4.36）%]比较，TTB各浓度组细胞增殖率分别为(73.00±2.76)%、(67.00±3.51)%、(52.00±2.83)%和(40.00±1.75)%，随着TBB浓度的增加，其对CAOV3细胞增殖的抑制呈剂量依赖性增强（P0.01）；细胞凋亡比例也呈剂量依赖性增加；与对照组比较，CK2α的表达逐渐降低，Bcl-2蛋白表达减少，Bax蛋白表达增加（P0.01）。结论 TBB可通过抑制抗凋亡蛋白Bcl-2的表达和促进促凋亡蛋白Bax的表达而促进卵巢癌CAOV3细胞凋亡。

Abstract

Objective To investigate the effect of protein kinase CK2 inhibitor 4

7-tetrabromobenzotriazole (TBB) on the proliferation and apoptosis of ovarian cancer CAOV3 cells and on the expression of Bcl-2 and Bax

and explore the possible mechanism. Methods CAOV3 cells were cultured in vitro and divided into a control group

a dimethyl sulfoxide (DMSO) group

and four TBB groups treated with different concentrations (12.5

50 and 75 μmol/L

respectively) of TBB. The proliferation of CAOV3 cells was measured by MTT assay

and the cell apoptosis was determined by flow cytometry. The changes of Bcl-2 and Bax proteins were detected by Western blotting. Results MTT assay showed that the proliferation rates of CAOV3 cells were (73.00±2.76)%

(67.00±3.51)%

(52.00±2.83)% and (40.00±1.75)%

respectively

in the 4 TBB groups

while that of the control group was (92.00±4.36)%. The cell proliferation inhibitory effect and cell apoptosis rate were increased as TBB concentration was increased (P0.01). The expression of Bcl-2 and CK2α proteins in CAOV3 cells treated with different concentrations of TBB were decreased

while Bax protein was increased as compared with the control group (P0.01). Conclusion TBB can induce apoptosis of ovarian cancer CAOV3 cells by down-regulating Bcl-2 protein and up-regulating Bax protein.

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