Objective      To investigate the effect of Notch signaling pathway on hyperoxia-induced oligodendrocyte maturation disorder in the brain tissue of neonatal mice.       Methods      Sixty-four C57BL/10J neonatal mice were randomly divided into air control group

air+DAPT (Notch signaling pathway blocker

10 mg/kg) group

hyperoxia group (hyperoxia exposure for 48 h)

and hyperoxia+DAPT group (hyperoxia exposure for 48 h after 10 mg/kg DAPT treatment). All neonatal mice were killed

and the brains were taken out to detect the mRNA expression changes of Notch1

Jagged1

Hes1 and Hes5 by real-time PCR in the 5th day after birth. In the 12th day after birth

oligodendrocytes were identified by immunofluorescent staining

and myelin basic protein (MBP) expression was detected by Western blotting.       Results      The hyperoxia group showed brain injury characterized by inhibition of brain development. There were significant differences in mRNA expressions of Notch1 and Jagged1 between the hyperoxia+DAPT group and the hyperoxia group (P0.05)

and the mRNA expressions of Hes1 and Hes5 in the hyperoxia-treated groups were significantly lower than those in the control group (P0.05). Higher expression of MBP was observed in the hyperoxia+DAPT group compared with the hyperoxial group (P0.05). Compared with the control group

the MBP expression was significantly decreased in the hyperoxia group (P0.05).       Conclusion      Notch signaling pathway involves in hyperoxia-induced brain damage in the neonatal mice

which is probably associated with the maturation of oligodendrocytes.