Radiosensitizing effect of 4-HPR on human cholangiocarcinoma cell line QBC939

朱浩然; 罗放; 朱方玉

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Radiosensitizing effect of 4-HPR on human cholangiocarcinoma cell line QBC939

更新时间：2026-01-09

- Radiosensitizing effect of 4-HPR on human cholangiocarcinoma cell line QBC939
- Journal of Army Medical University Vol. 37, Issue 17, Pages: 1761-1765(2015)
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  CLC： R730.55;R735.8;R914.4
- Published：2015
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朱浩然,罗放,朱方玉.4-HPR对人胆管癌QBC939细胞放射敏感性的增强作用[J].陆军军医大学学报(原第三军医大学学报),2015,37(17):1761-1765.

　Zhu Haoran,Luo Fang,Zhu Fangyu.Radiosensitizing effect of 4-HPR on human cholangiocarcinoma cell line QBC939[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(17):1761-1765.
朱浩然,罗放,朱方玉.4-HPR对人胆管癌QBC939细胞放射敏感性的增强作用[J].陆军军医大学学报(原第三军医大学学报),2015,37(17):1761-1765. DOI：

　Zhu Haoran,Luo Fang,Zhu Fangyu.Radiosensitizing effect of 4-HPR on human cholangiocarcinoma cell line QBC939[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(17):1761-1765. DOI：

摘要

目的探讨N-(4-羟基苯基）维生素甲酰胺（fenretinide，4-HPR）对人胆管癌QBC939细胞的放射敏感性。方法应用人胆管癌QBC939细胞进行培养，取对数生长期的细胞进行实验。随后采用MTT测定4-HPR对胆管癌QBC939细胞的抑制作用，计算出半数抑制浓度（IC

）。采用细胞克隆实验分析4-HPR 对QBC939细胞的放射增敏作用。采用流式细胞仪分析4-HPR对细胞凋亡的影响。Western blot检测相关蛋白的表达。结果 4-HPR对QBC939细胞具有抑制增殖作用，随剂量增加而增强。4-HPR与不同剂量的X线照射QBC939细胞后，与单纯照射组比较，4-HPR+照射组的细胞存活分数下降（P0.01）。单纯照射组、4-HPR照射组的平均致死剂量（D

）分别为2.72、1.93 Gy，准域剂量（D

）分别为2.06、0.94 Gy，4-HPR的放射增益比(SER)为1.41，说明4-HPR对胆管癌QBC939细胞有放射增敏作用。流式细胞仪检测结果显示，4-HPR作用于QBC939细胞后细胞凋亡率升高。bcl-2和caspase-3的Western blot检测结果显示，4-HPR明显增加了放射线诱导的胆管癌QBC939细胞的凋亡，表现为bcl-2的低表达和caspase-3的高表达。结论 4-HPR对胆管癌QBC939细胞有抑制作用和放射增敏作用，其放射增敏作用机制可能与诱导细胞凋亡有关。

Abstract

Objective To explore the radiosensitizing effect of fenretinide (4-HPR) on human cholangiocarcinoma cell line QBC939. Methods MTT assay was used to determine the half maximal inhibitory concentration (IC

) of 4-HPR to QBC939 cells. Radiosensitization of 4-HPR was measured by clone formation assay

and survival curve was described. Cell apoptosis was tested by flow cytometry. The apoptosis-assoc

iated proteins were analyzed by Western blotting. Results 4-HPR inhibited the proliferation of QBC939 cells in a concentration-dependent manner. Compared with the single radiotherapy group

the 4-HPR+radiotherapy group showed lower cell survival rate. The mean lethal radiation doses (D

) of the single radiotherapy group and 4-HPR+radiotherapy group were 2.72 Gy and 1.93 Gy

respectively

and the prospective domain doses (D

) were 2.06 Gy and 0.94 Gy

respectively. The gain ratio of radiotherapy (SER) of 4-HPR was 1.41. After 4-HPR treatment

the cell apoptosis was increased

showing low expression of bcl-2 and high expression of caspase-3. Conclusion 4-HPR inhibits the growth of QBC939 cells and enhances the radiosensitivity of QBC939 cells in vitro

which may be related with apoptosis.

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