Objective      To explore whether the effect of procadherin-10 (PCDH10) on inhibiting cell proliferation is r

elated with Wnt signaling in multiple myeloma (MM) and the underlying mechanism.       Methods      Human MM cell line KM3 was stably transfected with pcDNA3.1(+) or pcDNA3.1(+)PCDH10 by lipofectamine2000.The expression of PCDH10 gene was confirmed by RT-PCR and Western blottig. Different concentrations (0

5

10

15 μmol/mL and 20 μmol/mL）of LiCl was used to treat KM3 cells for 48 h

and then RT-PCR was adopted to determine the proper concentration of LiCl. CCK-8 assay was applied to examine the cell proliferation. Dual luciferase reporter assay was performed to assess the activity of LEF/TCF gene. Cell cycle was analyzed by flow cytometry. The expression of relative genes was detected by qPCR and Western blotting.       Results      The expression of PCDH10 gene was obviously increased after transfection. It was observed that the proper concentration of LiCl was 20 μmol/mL. PCDH10 over-expression highly inhibited the cell proliferation

even when the cells were treated with LiCl (P0.05). Dual luciferase reporter assay showed that the activity of LEF/TCF gene was significantly impaired by PCDH10 forced expression (P0.05). Flow cytometry revealed that PCDH10 arrested cell cycle in G

1

phase (P0.05)

and the cells treated with LiCl showed the same trend (P0.05). In addition

the expression of c-Myc and Cyclind1 were down-regulated at both mRNA and protein levels. The mRNA expression of β-catenin was not blocked evidently

buy the protein expression and nuclear accumulation of β-catenin were inhibited obviously even when the KM3 cells were treated with LiCl (P0.05).       Conclusion      PCDH10 can inhibit cell proliferation through inhibiting Wnt/β-catenin signaling in MM.