Panax notoginseng saponins suppresses mTOR signaling pathway in K562 cells

翟玮玮; 李玉云; 杨向荣; 于北凯; 王露; 李见

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Panax notoginseng saponins suppresses mTOR signaling pathway in K562 cells

更新时间：2026-01-09

- Panax notoginseng saponins suppresses mTOR signaling pathway in K562 cells
- Journal of Army Medical University Vol. 37, Issue 12, Pages: 1237-1242(2015)
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  CLC： R285.5;R73-354;R733.72
- Published：2015
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翟玮玮,李玉云,杨向荣,于北凯,王露,李见.三七总皂苷抑制K562细胞mTOR信号通路活性的实验研究[J].陆军军医大学学报(原第三军医大学学报),2015,37(12):1237-1242.

　Zhai Weiwei,Li Yuyun,Yang Xiangyong,Yu Beikai,Wang Lu,Li Jian.Panax notoginseng saponins suppresses mTOR signaling pathway in K562 cells[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(12):1237-1242.
翟玮玮,李玉云,杨向荣,于北凯,王露,李见.三七总皂苷抑制K562细胞mTOR信号通路活性的实验研究[J].陆军军医大学学报(原第三军医大学学报),2015,37(12):1237-1242. DOI：

　Zhai Weiwei,Li Yuyun,Yang Xiangyong,Yu Beikai,Wang Lu,Li Jian.Panax notoginseng saponins suppresses mTOR signaling pathway in K562 cells[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(12):1237-1242. DOI：

摘要

目的探讨三七总皂苷（Panax notoginseng saponins

PNS）对体外培养K562细胞 mTOR 信号通路相关分子表达及活性的影响。方法不同浓度的PNS（50～800 μg/mL）干预体外培养K562细胞24～72 h后，MTT比色法检测PNS对K562细胞增殖的抑制作用；AO/EB双荧光染色法观察细胞死亡及凋亡状况；RT-PCR检测mTOR、p70S6K、4E-BP1 mRNA 表达变化；Western blot检测mTOR、p70S6K、4E-BP1及p-mTOR、p-p70S6K、p-4E-BP1蛋白表达量的变化。结果与对照组相比，浓度为100～800 μg/mL 的PNS能够抑制K562细胞增殖（P0.05），促进K562细胞凋亡及死亡（P0.05），PNS对K562细胞72 h的IC

为（229.07±2.36）μg/mL；100、200、400 μg/mL PNS 作用于K562细胞72 h 后mTOR 蛋白表达量及mRNA 表达量降低（P0.05），且磷酸化蛋白p-mTOR (Ser2448)、p-p70S6K (Thr229/389)及p-4E-BP1(Thr37/46)表达水平也随着药物浓度的增加而降低（P0.05）。结论 PNS 能够抑制K562细胞 mTOR 信号通路的活性，这可能是PNS抑制K562细胞增殖的机制之一。

Abstract

Objective To investigate the effect of Panax notoginseng saponins (PNS) on the expression and activity of mTOR signaling pathway related molecules in K562 cells in vitro. Methods K562 cells were treated with different concentrations (50-800 μg/mL) of PNS for different periods of time (24-72 h). The cell proliferation was measured by MTT assay

and the cell death and apoptosis were observed by AO/EB double fluorescence staining. The mRNA expression of

mTOR

p70S6K and 4E-BP1 was examined by RT-PCR

and the protein expression of mTOR

p70S6K

4E-BP1 and their phosphorylated proteins (p-mTOR

p-p70S6K and p-4E-BP1) was detected by Western blotting. Results PNS (100-800 μg/mL) inhibited the proliferation of K562 cells (P0.05) and promoted cell apoptosis and death (P0.05)

and 72-h IC

was (229.07±2.36) μg/mL. After PNS (100

200 and 400 μg/mL) was applied to K562 cells for 72 h

the mRNA and protein expressions levls of mTOR were dose-dependently reduced (P0.05). In addition

the expression of p-mTOR (Ser2448)

p-p70S6K (Thr229/389) and p-4E-BP1 (Thr37/46) proteins was also decreased in a dose-dependent manner (P0.05). Conclusion PNS can inhibit the activity of mTOR signaling pathway in K562 cells

and this may be one of PNS-mediated cell proliferation inhibition mechanisms.

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