Objective      To determine the effect of clomipramine in U87 subcutaneous tumor model of nude mice.       Methods      U87 subcutaneous tumor model of nude mice was established

and the mice were divided into 3 groups: tumor model group

clomipramine groups

and temozolomide group. The clomipramine groups included 20

40 and 60 mg/kg sub-groups

and each group contains 6 tumor-bearing mice. The growth of tumor was observed. After the mice of the tumor model group were dead

the rest survival mice were sacrificed. The tumor size and survival time of tumor-bearing mice were compared

and the expression levels of translocator protein (TSPO)

Cytochrome C and Caspase-3 were detected.       Results      Compared with the tumor model group

clomipramine could inhibit the tumor growth

but the difference was not significant (P0.05). On the other hand

clomipramine could not significantly prolong the survival time of the tumor-bearing mice (P0.05). The expressions of TSPO and Cytochrome C were increased in the presence of high-concentration clomipramine (P0.05)

but Caspase-3 expression had no significantly statistical difference (P0.05).       Conclusion      Although clomipramine can inhibit the tumor growth and prolong the survival time of the tumor-bearing mice in vivo through activating the mitochondrial apoptotic pathways

the effect is not significant.