ARFI microbubble destruction improves targeted delivery of Fe<sub>3</sub>O<sub>4</sub> nanoparticles to subcutaneous transplantation tumor in SD rats

刘思思; 丁俊; 李锐; 陈朝晖

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ARFI microbubble destruction improves targeted delivery of Fe₃O₄ nanoparticles to subcutaneous transplantation tumor in SD rats

更新时间：2026-01-09

- ARFI microbubble destruction improves targeted delivery of Fe₃O₄ nanoparticles to subcutaneous transplantation tumor in SD rats
- Journal of Army Medical University Vol. 37, Issue 12, Pages: 1226-1231(2015)
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  CLC： R73-354;R730.41;R981
- Published：2015
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刘思思,丁俊,李锐,陈朝晖.ARFI破坏微泡增强Fe3O4纳米粒子在大鼠皮下移植瘤靶向递送的实验研究[J].陆军军医大学学报(原第三军医大学学报),2015,37(12):1226-1231.

　Liu Sisi,Ding Jun,Li Rui,Chen Zhaohui.ARFI microbubble destruction improves targeted delivery of Fe3O4 nanoparticles to subcutaneous transplantation tumor in SD rats[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(12):1226-1231.
刘思思,丁俊,李锐,陈朝晖.ARFI破坏微泡增强Fe3O4纳米粒子在大鼠皮下移植瘤靶向递送的实验研究[J].陆军军医大学学报(原第三军医大学学报),2015,37(12):1226-1231. DOI：

　Liu Sisi,Ding Jun,Li Rui,Chen Zhaohui.ARFI microbubble destruction improves targeted delivery of Fe3O4 nanoparticles to subcutaneous transplantation tumor in SD rats[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(12):1226-1231. DOI：

摘要

目的制备一种具有磁共振显像功能的Fe

纳米粒子，通过声脉冲辐射成像（acoustic radiation force impulse，ARFI）辐照脂质微泡（microbubbles，MBs），探讨ARFI辐照微泡对Fe

纳米粒子在肿瘤组织分布的影响。方法采用高温水热法制备Fe

纳米粒子

检测其形态、大小、分布等，观察其体外磁共振显像效果。选取60只SD雌性大鼠，体质量为170~200 g，制备Walker256皮下移植瘤模型，分为6组（n=10）：单纯ARFI辐照组、单纯MBs组、ARFI辐照MBs组、单纯Fe

纳米粒子组、ARFI辐照Fe

纳米粒子组、ARFI辐照MBs和Fe

纳米粒子组。微泡0.2 mL及5 mg/kg Fe

纳米粒子经大鼠尾静脉推注，ARFI辐照条件为探头间隔5 s辐照肿瘤部位，累计辐照5 min。将处理后的SD大鼠肿瘤部位行MRI扫描观察肿瘤组织信号变化；处死SD大鼠，取组织标本行病理学分析。结果制备的Fe

纳米粒子形态规则，粒径分布均匀，具有磁共振显像功能。SD大鼠肿瘤组织普鲁士蓝染色结果为单纯Fe

纳米粒子组、ARFI辐照Fe

纳米粒子组、ARFI辐照MBs和Fe

纳米粒子组均可见点状蓝染颗粒。其中ARFI辐照MBs和Fe

纳米粒子组蓝染颗粒计数明显多于其余2组（P0.05）。SD大鼠肿瘤部位磁共振成像结果为单纯Fe

纳米粒子组、ARFI辐照Fe

纳米粒子组、ARFI辐照MBs和Fe

纳米粒子组经相应处理后T

*WI均可见肿瘤内部低信号部分增加。结论 ARFI辐照MBs能够有效地提高Fe

纳米粒子在SD大鼠皮下移植瘤组织的分布，增强Fe

纳米粒子在活体肿瘤内的靶向递送效果。

Abstract

Objective To prepare Fe

nanoparticles and explore their in vitro magnetic resonance (MRI) effect

and investigate the impact of acoustic radiation force impulse (ARFI) microbubble (MB) destruction on targeted delivery of Fe

nanoparticles to subcutaneous transplantation tumor in SD rats. Methods High-temperature hydrothermal reaction was used to prepare Fe

nanoparticles

and the particle morphology

size

and distribution were assessed. Fe

nanoparticles were applied to perform MRI in vitro. Sixty female SD rats (170-200 g) were prepared to establish the subcutaneous transplantation tumor using Walker256 cells

and the rats were randomly divided into 6 groups (n=10)

which were treated by ARFI irradiation

MB injection

ARFI MB destruction

nanoparticle injection

nanoparticle injection and ARFI irradiation

and Fe

nanoparticle injection and ARFI MB destruction

respectively. MBs and Fe

nanoparticles were given by intravenous injection. The dose of MBs was 0.2 mL per rat

and the dose of Fe

nanoparticles was 5 mg/kg. ARFI irradiation parameters were exposure duration 5 s

then pause 5 s

and total 5 min. MRI was employed to observe the signal change of tumor before and afterFe

nanoparticle injection and AFRI irradiation by T

*-weighted imaging (T

*WI). The rats were killed for pathological assay after management. Results The prepared Fe

nanoparticles were observed with regular spherical morphology and narrow size distribution

and had significant superparamagnetic properties for imaging. Prussian blue staining represented the distri

bution of Fe

nanoparticles in the tumor. The Fe

nanoparticle injection group

nanoparticle injection and ARFI irradiation group

and Fe

nanoparticle injection and ARFI MB destruction group were observed with blue stained particles

and the blue stained particles in the Fe

nanoparticle injection and ARFI MB destruction group were statistically more than those in the other 2 groups (P0.05). Increased hypointense areas were observed in the tumor of the Fe

nanoparticle injection group

nanoparticle injection and ARFI irradiation group

and Fe

nanoparticle injection and ARFI MB destruction group. Conclusion ARFI MB destruction improves Fe

nanoparticle distribution in the subcutaneous transplantation tumor of the SD rats

and thus enhances in vivo tumor targeted delivery of Fe

nanoparticles.

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Related Institution

Department of Radiology

Chongqing Clinical Research Centre of Imaging and Nuclear Medicine

Department of Pathology， Daping Hospital， Army Medical University

Department of Radiology， the First Affiliated Hospital of Chongqing Medical University

Department of Radiology，First Affiliated Hospital， Army Medical University （Third Military Medical University）

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ARFI microbubble destruction improves targeted delivery of Fe3O4 nanoparticles to subcutaneous transplantation tumor in SD rats

DOI：

摘要

Abstract

关键词

Keywords

references

ARFI microbubble destruction improves targeted delivery of Fe₃O₄ nanoparticles to subcutaneous transplantation tumor in SD rats