Correlation of glutathione S-transferase polymorphisms with sperm mitochondrial membrane potential in idiopathic male infertility

唐开发; 张洋; 赵伊立; 王新阳; 邹铁军; 孙发; 邢俊平

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Correlation of glutathione S-transferase polymorphisms with sperm mitochondrial membrane potential in idiopathic male infertility

更新时间：2026-01-09

- Correlation of glutathione S-transferase polymorphisms with sperm mitochondrial membrane potential in idiopathic male infertility
- Journal of Army Medical University Vol. 37, Issue 15, Pages: 1539-1542(2015)
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  CLC： R329.24;R394.3;R698.2
- Published：2015
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唐开发,张洋,赵伊立,王新阳,邹铁军,孙发,邢俊平.谷胱甘肽S-转移酶基因多态性与特发性男性不育症精子线粒体膜电位的相关性[J].陆军军医大学学报(原第三军医大学学报),2015,37(15):1539-1542.

　Tang Kaifa,Zhang Yang,Zhao Yili,Wang Xinyang,Zou Tiejun,Sun Fa,Xing Junping.Correlation of glutathione S-transferase polymorphisms with sperm mitochondrial membrane potential in idiopathic male infertility[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(15):1539-1542.
唐开发,张洋,赵伊立,王新阳,邹铁军,孙发,邢俊平.谷胱甘肽S-转移酶基因多态性与特发性男性不育症精子线粒体膜电位的相关性[J].陆军军医大学学报(原第三军医大学学报),2015,37(15):1539-1542. DOI：

　Tang Kaifa,Zhang Yang,Zhao Yili,Wang Xinyang,Zou Tiejun,Sun Fa,Xing Junping.Correlation of glutathione S-transferase polymorphisms with sperm mitochondrial membrane potential in idiopathic male infertility[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(15):1539-1542. DOI：

摘要

目的该研究旨在探讨谷胱甘肽S-转移酶基因M1、T1及P1（GSTM1、T1及P1）基因多态性与特发性男性不育症患者精子线粒体膜电位（mitochondrial membrane potential，MMP）的相关性。方法该研究包括246例特发性少弱精子性男性不育症患者。采用聚合酶链反应（PCR），聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分别对GSTM1、T1和P1基因进行分型。采用5

5′

6′-四氯-1

1′

3′-四乙基苯并咪唑羰花青碘化物（JC-1）染色剂标记流式细胞仪检测精子线粒体膜电位（MMP）进行检测分析。结果 GSTM1及T1基因缺失型组特发性少弱精子性男性不育症患者正常MMP精子百分比显著低于野生型组（P0.01）；且二者间具有协同效应。GSTP1基因突变型组正常MMP精子百分比与GSTP1基因野生型组想比较，无统计学差异（P=0.085）。结论 GSTM1、T1基因缺失型是特发性男性不育症精子MMP的危险因素。

Abstract

Objective To investigate the correlation of glutathione S-transferase M1

T1 and P1 gene (GSTM1

GSTT1 and GSTP1) polymorphisms with sperm mitochondrial membrane potential (MMP) in idiopathic male infertility. Methods A total of 246 patients with idiopathic male infertile admitted in the First Affiliated Hospital of Medical College

Xi’an Jiaotong University from September 2008 to June 2012 were enrdled in this study. Polymerase chain reaction (PCR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were used to identify the GSTM1

GSTT1 and GSTP1 genotypes in the sperm . Then flow cytometry using 5

5′

6′-tetrachloro-1

1′

3′-tetraethylbenzimidazolylcarbocyanine (JC-1) as staining agent was employed to detect the sperm MMP. Results The percentage of normal sperm MMP was significantly lower in the GSTM1 and GSTT1 deletion groups than the wild type group in idiopathic male infertile patients (P0.01)

and the 2 type of gene detection had synergistic effect. However

there was no significant difference in the percentage of normal sperm MMP between the GSTP1 mutation type group and the wild type group (P=0.085). Conclusion GSTM1 and GSTT1 deletions are risk factors for the sperm MMP in the idiopathic male infertile patients.

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