MicroRNA-193b down-regulates bcr/abl in K562 cells

舒燕; 齐杰玉; 王灿蔚; 于淑静; 陶崑

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MicroRNA-193b down-regulates bcr/abl in K562 cells

更新时间：2026-01-09

- MicroRNA-193b down-regulates bcr/abl in K562 cells
- Journal of Army Medical University Vol. 37, Issue 19, Pages: 1955-1959(2015)
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  CLC： R394.3;R730.23;R733.72
- Published：2015
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舒燕,齐杰玉,王灿蔚,于淑静,陶崑.MicroRNA-193b对K562细胞bcr/abl表达的影响[J].陆军军医大学学报(原第三军医大学学报),2015,37(19 ):1955-1959.　Shu Yan,Qi Jieyu,Wang Canwei,Yu Shujing,Tao Kun.MicroRNA-193b down-regulates bcr/abl in K562 cells[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(19 ):1955-1959.

舒燕, 齐杰玉, 王灿蔚, et al. MicroRNA-193b down-regulates bcr/abl in K562 cells[J]. Journal of Army Medical University, 2015, 37(19): 1955-1959.
舒燕,齐杰玉,王灿蔚,于淑静,陶崑.MicroRNA-193b对K562细胞bcr/abl表达的影响[J].陆军军医大学学报(原第三军医大学学报),2015,37(19 ):1955-1959.　Shu Yan,Qi Jieyu,Wang Canwei,Yu Shujing,Tao Kun.MicroRNA-193b down-regulates bcr/abl in K562 cells[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(19 ):1955-1959. DOI：

舒燕, 齐杰玉, 王灿蔚, et al. MicroRNA-193b down-regulates bcr/abl in K562 cells[J]. Journal of Army Medical University, 2015, 37(19): 1955-1959. DOI：

摘要

目的用Ad-pre-miRNA-193b重组腺病毒感染K562细胞，探讨miR-193b在细胞中过表达对bcr/abl表达的影响。方法将前期构建的Ad-pre-miRNA-193b重组腺病毒感染K562细胞后，采用荧光定量PCR（qRT-PCR）检测miR-193b的表达；qRT-PCR检测bcr/abl融合基因的表达；Western blot检测BCR/ABL融合蛋白的表达；CCK-8检测K562细胞的增殖情况，流式细胞术（FCM）检测K562细胞凋亡率。结果与对照比较，K562细胞感染Ad-pre-miRNA-193b重组腺病毒后，miR-193b表达明显升高；bcr/abl融合基因及BCR/ABL融合蛋白表达明显下调；K562细胞增殖能力明显降低（P0.05），凋亡水平明显升高（P0.05）。结论 miR-193b可下调bcr/abl表达，抑制K562细胞的生物学效应，过表达miR-193b能作为治疗CML的有效手段。

Abstract

Objective To determine the effect of miR-193b over-expression on the expression of bcr/abl in chronic myelocytic leukemia K562 cells by transfection of recombinant adenovirus of Ad-pre-microRNA-193b. Methods After K562 cells were infected by Ad-pre-microRNA-193b virus

the expression of miR-193b was detected by real-time PCR. The expression of bcr/abl at mRNA and protein levels was detected by qRT-PCR and Western blotting. Cell proliferation was measured by CCK-8 assay

and cell apoptosis was tested by flow cytometry. Results Compared with control cells

the level of microRNA-193b was significantly up-regulated in K562 cells after Ad-pre-microRNA-193b infection. The expression of bcr/abl was significantly decreased

and BCR/ABL fusion protein was decreased too. The virus infection resulted in significant inhibition in cell proliferation and obvious improvement in apoptotic rate (both P0.05). Conclusion miR-193b down-regulates the expression of bcr/abl and inhibits the biological effects of K562 cells. The over-expression of miR-193b may be an effective target in the treatment of chronic myelogenous leukemia.

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