Objective       To investigate the effect of lentivirus-mediated large tumor suppressor gene 1 (LATS1) overexpression on the cell proliferation

apoptosis and downstream oncogene Yes-associated protein (YAP) in human clear cell renal cell carcinoma (ccRCC) cell line 786-0.       Methods      LATS1 gene was introduced into 786-0 cells via lentiviral vector. The mRNA expr

ession levels of LATS1 and YAP in Hippo-YAP signaling pathway were detected by RT-PCR

while the protein levels were detected by Western blotting. The cell apoptosis and cell cycle were analyzed by flow cytometry (FCM)

and the cell proliferation inhibition was assayed by cell proliferation and toxicity detection reagent (cell counting kit-8).       Results      After 786-0 cells were transfected with LATS1 gene

the expression of LATS1 at mRNA and protein levels was significantly increased (P0.05)

while the expression of YAP at mRNA and protein levels was markedly decreased (P0.05). After lentiviral-mediated LATS1 overexpression

the cell cycle was arrested at G

1

phase (P0.05)

the cell proliferation was obviously inhibited (P0.05)

and the cell apoptosis was increased significantly (P0.05).       Conclusion       Lentivirus-mediated LATS1 overexpression down-regulates the expression of YAP

inhibits the cell proliferation of 786-0 cells

induces the cell apoptosis and blocks the cell cycle at G

1

phase. The occurrence and development of RCC may be associated with expression disorder of LATS1 and YAP.