Bone marrow stromal antigen 2 inhibits replication and release of hepatitis B virus

朱素菲; 贺巧; 胡琴; 黄英; 陈维贤; 李月

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Bone marrow stromal antigen 2 inhibits replication and release of hepatitis B virus

更新时间：2026-01-09

- Bone marrow stromal antigen 2 inhibits replication and release of hepatitis B virus
- Journal of Army Medical University Vol. 37, Issue 17, Pages: 1719-1714(2015)
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  CLC： R373.21;{R392.13};R977.1
- Published：2015
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朱素菲,贺巧,胡琴,黄英,陈维贤,李月.骨髓基质细胞抗原2抑制乙肝病毒复制与释放[J].陆军军医大学学报(原第三军医大学学报),2015,37(17):1719-1714.

　Zhu Sufei,He Qiao,Hu Qin,Huang Ying,Chen Weixian,Li Yue.Bone marrow stromal antigen 2 inhibits replication and release of hepatitis B virus[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(17):1719-1714.
朱素菲,贺巧,胡琴,黄英,陈维贤,李月.骨髓基质细胞抗原2抑制乙肝病毒复制与释放[J].陆军军医大学学报(原第三军医大学学报),2015,37(17):1719-1714. DOI：

　Zhu Sufei,He Qiao,Hu Qin,Huang Ying,Chen Weixian,Li Yue.Bone marrow stromal antigen 2 inhibits replication and release of hepatitis B virus[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(17):1719-1714. DOI：

摘要

目的研究骨髓基质细胞抗原2(bone marrow stromal antigen 2

BST-2)对乙型肝炎病毒(hepatitis B virus，HBV)复制与释放的影响。方法以不同浓度干扰素处理人肝癌细胞株HepG2.2.15，Western blot和荧光定量 PCR检测BST-2蛋白水平和RNA水平；分别用构建的BST-2真核表达质粒pCDNA3.1-BST2-FLAG和BST-2拮抗蛋白HIV Vpu真核表达质粒，转染HepG2.2.15细胞，酶联免疫吸附实验(ELISA)检测细胞培养液乙肝病毒表面抗原（HBsAg）和乙肝病毒e抗原(HBeAg)

荧光定量PCR检测HBV mRNA，Southern blot检测HBV DNA。结果转染pCDNA3.1-BST2-FLAG后，HBV mRNA降低26%，细胞内HBV DNA降低31%，细胞培养液中HBV DNA降低33%(P0.05)，细胞培养液中HBsAg下降至82%，HBeAg下降至77%(P0.05)；加入Vpu蛋白后，BST-2的抑制作用受到对抗，HBV mRNA恢复至92%，细胞内HBV DNA恢复至93%，细胞培养液中HBV DNA升至98%，细胞培养液中HBsAg恢复至102%，HBeAg恢复至99%，HBV复制与释放明显增强。结论 BST-2对HBV复制与释放具有抑制作用，该抑制作用可被HIV Vpu蛋白拮抗。

Abstract

Objective To determine the effect of interferon-induced protein bone marrow stromal antigen 2 (BST-2) on the replication and release of hepatitis B virus (HBV). Methods Human hepatoma cell line HepG2.2.15 was treated with different concentrations of interferon

and the expression level of BST-2 was determined by Western blotting and quantitative PCR. Eukaryotic expression plasmid pCDNA3.1-BST2-FLAG was constructed and transferred into HepG2.2.15 cells. The levels of HBV surface antigen (HBsAg) and HBV e antigen (HBeAg) in cell cultures were detected by enzyme-linked immunosorbent assay (ELISA). HBV mRNA levels were detected by fluorescence quantitative PCR

while HBV DNA levels were detected by Southern blotting. Then

BST-2 antagonist protein

HIV Vpu

was added to inhibit BST-2

and HBsAg

HBeAg

HBV mRNA and HBV DNA levels were detected with the same methods as above. Results After transfection of HepG2.2.15 cells with pCDNA3.1-BST2-FLAG

HBsAg and HBeAg levels in cell cultures and intracellular HBV mRNA and DNA levels were decreased significantly (P0.05). After HIV Vpu protein was added

the inhibitory effect of BST2 was antagonized

and HBV replication was recovered partially. Conclusion BST-2 inhibits the replication and release of HBV

and the inhibitory effect can be antagonized with HIV Vpu protein.

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